Establishment of a neutralization test involving reporter gene-expressing virus-like particles of tick-borne encephalitis virus
スポンサーリンク
概要
- 論文の詳細を見る
Previously, a system for packaging tick-borne encephalitis virus (TBEV) subgenomic replicon RNAs into single-round infectious virus-like particles (VLPs) was developed. In the present study, VLPs were applied to measuring the levels of neutralizing antibodies against TBEV as an alternative to performing neutralization tests with live virus. As markers of VLP infection, the genes for GFP and luciferase were inserted into the TBEV replicon, which was then packaged into VLPs. The reporter genes were expressed in cells that were infected with the VLPs, and this infection was inhibited by neutralizing antibodies to TBEV. Serum samples from wild rodents were used to evaluate the neutralization test using VLPs. All the sera that were positive in the conventional neutralization test were also found to be positive in the neutralization test using VLPs, and there were highly significant correlations between the neutralization titres obtained using the native virus and those using VLPs. These results indicate that VLPs that express reporter genes represent a useful and safe alternative to conventional neutralization testing using live virus.
- Elsevier B.V.の論文
著者
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Itoh T
Iclas Monitoring Center Central Institute For Experimental Animals
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Kumada Hiromitsu
冲中記念成人病研究所
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Kumada Hiromitsu
国家公務員共済組合連合会虎の門病院 肝臓科
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Kumada Hiromitsu
国家公務員共済組合連合会虎の門病院 肝臓内科
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Kariwa Hiroaki
Hokkaido Univ. Sapporo Jpn
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Kariwa Hiroaki
北海道大学 獣医研究科
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Kumada H
Department Of Hepatology Toranomon Hospital
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Takashima I
Hokkaido Univ. Sapporo Jpn
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Takashima Ikuo
Hokkaido Univ. Hokkaido Jpn
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Koga Hiroyuki
Department Of Hepatology Toranomon Hospital
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Kariwa H
Hokkaido Univ. Sapporo Jpn
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