General characteristics of the superprecipitation reaction of rat crude uterine myosin B: Effects of L-methionine and/or transmethylation blockers on the reaction.

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概要

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• The characteristics of superprecipitation of uterine myosin B from 17β-estradiol-3-benzoate-treated rats and the effects of L-methionine and/or 3-deazaadenosine (3-DAA) plus homocysteine thiolactone (HCTL) on the reaction were investigated. The slope and plateau phases of the superprecipitation were dependent on ATP, calcium and magnesium. The calmodulin antagonists, trifluoperazine and N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide, inhibited the slope and plateau phases, but not the basal superprecipitation. SDS-polyacrylamide gel electrophoresis of the precipitate obtained in complete medium showed the presence of actin and myosin. Addition of 3 mM L-methionine had little affect on either the slope or plateau phases of the basal or calcium-dependent superprecipitation, while 500 μM 3-DAA plus 1 mM HCTL caused only 9 to 20% decrease in the calcium-dependent superprecipitation in the presence of 0.1 mM or 1 mM ATP. These findings suggest that the calcium-dependent superprecipitation of rat uterine myosin B reflected the contractile response of uterine muscle, and that L-methionine and/or 3-DAA plus HCTL did not affect the calcium-dependent superprecipitation of uterine myosin B.

• 公益社団法人 日本薬理学会の論文

著者

• Ichida Seiji

  Department Of Biological Chemistry School Of Pharmaceutical Sciences Kinki University

• Okazaki Yuko

  Department Of Allergy And Immunological Diseases Tokyo Metropolitan Komagome Hospital

• Sobue Kenji

  Department Of Neurochemistry Biomedical Research Center

• Yoshioka Kazuo

  Department Of Pediatrics Kinki University School Of Medicine

• Tanaka Toshihiko

  Department Of Dermatology Faculty Of Medicine Hiroshima University

• Ariyoshi Shunji

  Department Of Pharmacology Saga Medical School

• ICHIDA Seiji

  Departments of Biological Chemistry, Faculty of Pharmacy, Kinki University

• SOBUE Kenji

  Department of Neurochemistry and Neuropharmacology, Institute of Higher Nervous Activity, Osaka University Medical School

• ARIYOSHI Shunji

  Department of Biological Chemistry, Faculty of Pharmacy, Kinki University

• OKAZAKI Yuko

  Departments of Instrumental Analysis, Faculty of Pharmacy, Kinki University

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