The Inhibitory Effect of the Toxic Fraction from Sea Urchin (Toxopneustes pileolus) Venom on ^<45>Ca^<2+> Uptake in Crude Synaptosome Fraction from Chick Brain
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概要
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The effects of toxic peakes (P-I, P-II and P-III eluted from Sephadex G-200 column) from the sea urchin Toxopneustes pileolus on time-dependent ^<45>Ca^<2+> uptake in chike P_2 fraction (crude synaptosome fraction) were studied under physiological ionic conditions. Time-dependent ^<45>Ca^<2+> uptake was inhibited by P-II and P-III, but not by P-I, P-II had the greatest inhibitory effect. The inhibitory effect of P-II was not due to the inhibition of ^<45>Ca^<2+> binding on P_2 fraction, because P-II did not affect ^<45>Ca^<2+> binding in osmotic-shocked P_2 fraction. P-II did not affect KCl-stimulated ^<45>Ca^<2+> uptake in P_2 fraction, (Ca^<2+>-Mg-<2+>)-ATPase activity in the synaptic plasma membrane (SPM) fraction, or (Na^+-K^+)-ATPase and Mg^<2+>-ATPase activities on osmotically-shocked P_2 fraction. In contrast, the Na^+/Ca^<2+> exchanger blocker 2', 4'-dichlorobenzamil (DCB; 100μM), with a poor specificity, inhibited not only time-dependent ^<45>Ca^<2+> uptake but also KCl-stimulated ^<45>Ca^<2+> uptake, (Ca^<2+>-Mg^<2+>)-ATPase, Mg^<2+>-ATPase and (Na^+-K^+)-ATPase. Involvement of Na^+-Ca^<2+> exchanger in the time-dependent ^<45>Ca^<2+> uptake was ruled out, since in was not inhibited by replacement of Na^+ with Li^+ in reactio medium. These results suggested that the inhibition by P-II on time-dependent ^<45>Ca^<2+> uptake appeared to be more specific than the commercially available Na^+/Ca^<2+> exchanger blocker DCB, although the mechanism is not clear yet.
- 社団法人日本薬学会の論文
- 1999-12-15
著者
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Zhang Yu-an
Department Of Biological Chemistry Faculty Of Pharmaceutical Sciences Kinki University
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Zhang Yu-an
近畿大学 薬 生化
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WADA Tetsuyuki
Department of Biological Chemistry, School of Pharmaceutical Sciences, Kinki University
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ICHIDA Seiji
Department of Biological Chemistry, School of Pharmaceutical Sciences, Kinki University
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Iwasaki Yasunori
Department of Small Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen Universit
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Ichida S
Kinki Univ. Osaka Jpn
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Ichida Seiji
Department Of Biological Chemistry School Of Pharmaceutical Sciences Kinki University
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Wada Tetsuyuki
Department Of Biological Chemistry Faculty Of Pharmaceutical Sciences Kinki University
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Wada Tetsuyuki
Department Of Biological Chemistry School Of Pharmaceutical Sciences Kinki University
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Wada Tetsuyuki
Faculty Of Pharmaceutical Sciences Kinki University
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NAKAGAWA Hideyuki
Department of Electrical and Electronics Engineering, Fukui University
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Iwasaki Yasunori
Department Of Biological Chemistry Faculty Of Pharmaceutical Sciences Kinki University
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Ichida S
Department Of Biological Chemistry Faculty Of Pharmaceutical Sciences Kinki University
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Nakagawa Hideyuki
Department Of Anesthesiology Saitama International Medical Center Saitama Medical University
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Nakagawa Hideyuki
Department Of Life Science Faculty Of Integrated Arts And Sciences The University Of Tokushima
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