Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from a Nitrite-Oxidizing Chemoautotroph, Nitrobacter agilis ATCC 14123 : Purification and Properties

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概要

• 論文の詳細を見る

• Nitrite-oxidizing chemoautotrophic Nitrobacter agilis ATCC 14123 exhibited remarkedly strong ribulose-1,5-bisphosphate carboxylase (RuBisCO) activity. RuBisCO[EC4.1.1.39] was purified as an electrophoretically homogeneous protein. The molecular mass of the enzyme was estimated to be about 480 kDa by gel filtration, suggesting that the enzyme consisted of two different subunits [Large (L) : 50 kDa, Small (S) : 10 kDa], as demonstrated by SDS-PAGE. This confirmed that the enzyme has a L_8S_8 structure. The K_m values of the enzyme for RuBP, NaHCO_3,Mg^<2+> were estimated to be 0.039 mM, 6.39 mM and 1.36 mM, respectively. The optimum pH and temperature for its activity were approximately 8.0 and 40℃, respectively. The enzyme was stable up to 45℃ and in a pH range of 7.0-9.0 (4℃, 48h). The enzyme activity was inhibited by Mo^<2+>, Cu^<2+>, Hg^<2+>, N-ethylmaleimide, p-chloromercuribenzoate and SDS (1 mM). The N-terminal amino acid sequence of the large subunit is AVKSYQAGVTQYRQSYWQPDYMPL, and that of the small subunit is AVQAYRSLKKYETFSYLPQ.

• 社団法人日本生物工学会の論文
• 1996-03-25

著者

• MASUKO Takashi

  Department of Cell Biology, School of Pharmaceutical Sciences, Kinki University

• Masuko Takashi

  近畿大学 薬学部細胞生物学

• Tachibana Makoto

  Interdisciplinary Graduate School Of Science And Engineering Tokyo Institute Of Technology:(present

• Masuko Takashi

  Laboratory Of Cell Biology School Of Pharmaceutical Sciences Kinki University

• Masuko Takashi

  Departments Of Molecular Biology Pharmaceutical Institute Tohoku University

• Masuko Takashi

  Department Of Agricultural And Biological Chemistry College Of Bioresource Sciences Nihon University

• TAKAHASHI Reiji

  Department of Agricultural and Biological Chemistry, College of Bioresource Sciences, Nihon Universi

• TOKUYAMA Tatsuaki

  Department of Agricultural and Biological Chemistry, College of Bioresource Sciences, Nihon Universi

• FUJIOKA TOMOKO

  Department of Agricultural Chemsitry, College of Agriculture and Veterinary Medicine, Nihon Universi

• Takahashi Reiji

  Department Of Agricultural Chemistry College Of Agriculture And Veterinary Medicine Nihon University

• Takahashi Reiji

  Department Of Agricultural And Biological Chemistry College Of Bioresource Sciences Nihon University

• Tokuyama Tatsuaki

  College Of Bioresource Sciences Nihon University

• Tokuyama Tatsuaki

  Department Of Agricultural And Biological Chemistry College Of Bioresource Sciences Nihon University

• Tokuyama T

  College Of Bioresource Sciences Nihon University

• Fujioka T

  Chuo Univ. Tokyo Jpn

• Takahashi R

  Tamagawa Univ. Tokyo Jpn

• Fujioka Tomoko

  Department Of Agricultural Chemsitry College Of Agriculture And Veterinary Medicine Nihon University

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