Identification of Genus Nitrosovibrio, Ammonia-Oxidizing Bacteria, by Comparison of N-Terminal Amino Acid Sequences of Phosphoglycerate Kinase(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
Nitrosospira, Nitrosolobus, and Nitrosovibrio, three genera of ammonia-oxidizing bacteria (AOB), are morphologically distinct from each other (Nitrosospira : spiral, Nitrosolobus : lobate, Nitrosovibrio : slender curved rods). However, these three genera cannot be classified using the phylogenetic tree based on 16S rDNA sequences. Phosphoglycerate kinase (PGK) was purified from 10 AOB strains as an index for identification, and its enzymatic properties were investigated. No distinction could be made between these three genera of AOB, because there was no notable difference in the molecular weight, K_m, or other properties of their PGKs. However, the N-terminal amino acid residues of PGK of Pro (P)-1, Ile (I) -6 and Asp (D) -12 have sequences characteristic of genus Nitrosovibrio (three strains) in four AOB genera. Therefore, it is proved possible to use the differences in the sequence to identify individual strains of the genus Nitrosovibrio.
- 社団法人日本生物工学会の論文
- 2004-11-25
著者
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Tanaka Tomohiro
Department Of Agricultural And Biological Chemistry College Of Bioresource Sciences Nihon University
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SATOH Masayuki
Department of Nuclear Medicine, Hokkaido University Graduate School of Medicine
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TAKAHASHI Reiji
Department of Agricultural and Biological Chemistry, College of Bioresource Sciences, Nihon Universi
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TOKUYAMA Tatsuaki
Department of Agricultural and Biological Chemistry, College of Bioresource Sciences, Nihon Universi
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IDA TAKESHI
Department of Surgery, Shimane Medical University
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Ida Takeshi
College Of Bioresource Sciences Nihon University
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Ida Takeshi
Department Of Surgery Shimane Medical University
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Takahashi Reiji
College Of Bioresource Sciences Nihon University
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Takahashi Reiji
Department Of Agricultural And Biological Chemistry College Of Bioresource Sciences Nihon University
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Tokuyama Tatsuaki
College Of Bioresource Sciences Nihon University
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Tokuyama Tatsuaki
Department Of Agricultural And Biological Chemistry College Of Bioresource Sciences Nihon University
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Tokuyama T
College Of Bioresource Sciences Nihon University
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Satoh Masayuki
Department Of Biology Faculty Of Science Toyama University
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Tanaka Tomohiro
College Of Bioresource Sciences Nihon University
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YABE RYUICHI
College of Bioresource Sciences, Nihon University
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YABE RYUICHI
Department of Agricultural and Biological Chemistry, College of Bioresource Sciences, Nihon Universi
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Ida Tetsuro
College Of Bioresource Sciences Nihon University
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Yabe Ryuichi
College Of Bioresource Sciences Nihon University
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Satoh Masayuki
Department Of Agricultural And Biological Chemistry College Of Bioresource Sciences Nihon University
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