Purification and Some Properties of Citrate Synthase from Ammonia : Oxidizing Chemoautotrophic Nitrosomonas europaea ATCC 25978
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概要
- 論文の詳細を見る
Ammonia-oxidizing chemoautotrophic Nitrosomonas europaea ATCC 25978 exhibited remarkable oxaloacetate-oxidative activity. Citrate(si)-synthase [EC 4.1.3.7.] was purified as an electrophoretic homogeneous protein. The molecular weight of the enzyme was estimated to be about 295,000 (α_xβ_y) by gel filtration, suggesting that the enzyme consisted of two different subunits (α ; 65,000,β ; 75,000), as demonstrated by SDS-PAGE. The N-terminal sequences of α and β type enzyme proteins were Ala-Leu-Val-Ser-Leu-Arg-Gln-Leu-Leu. The isoelectric point of the enzyme was pH 5.2. The enzyme was stable up to 35℃ and in a pH range between 6.0-8.0. The optimum pH for the reaction was 6.5-8.5 and the optimum temperature was 30-40℃. The apparent K_m Values for oxaloacetate and acetyl-CoA were about 0.025 and 0.080 mM, respectively. The enzyme was not inhibited by ATP (1mM), NADH (1mM) or 2-oxoglutamic acid (10mM). Activation by metal ions (0.1mM) was not observed.
- 日本微生物生態学会の論文
- 1992-11-30
著者
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KONDO Hiroshi
Department of Civil Engineering, School of Engineering
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TAKAHASHI Reiji
Department of Agricultural and Biological Chemistry, College of Bioresource Sciences, Nihon Universi
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TOKUYAMA Tatsuaki
Department of Agricultural and Biological Chemistry, College of Bioresource Sciences, Nihon Universi
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Takahashi Reiji
Department Of Agricultural Chemistry College Of Agriculture And Veterinary Medicine Nihon University
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Takahashi Reiji
Department Of Agricultural And Biological Chemistry College Of Bioresource Sciences Nihon University
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Tokuyama Tatsuaki
College Of Bioresource Sciences Nihon University
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Tokuyama Tatsuaki
Department Of Agricultural And Biological Chemistry College Of Bioresource Sciences Nihon University
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Tokuyama T
College Of Bioresource Sciences Nihon University
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Ohmori Takashi
Department of Health and Dietary Science, Research and Development Center, Nippon Meat Packers, Inc.
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Ohmori Takashi
Department Of Agricultural Chemistry College Of Agriculture And Veterinary Medicine Nihon University
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Takahashi R
Tamagawa Univ. Tokyo Jpn
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Kondo Hiroshi
Department Of Agricultural Chemistry College Of Agriculture And Veterinary Medicine Nihon University
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