A Novel Growth Factor Highly Mitogenic on Immortalized Cells from Rat Serum : Purification and Its Properties
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概要
- 論文の詳細を見る
A growth factor that is highly mitogenic on BALB/c 3T3 cells and exists exclusively in rat and mouse sera was purified from rat serum by heparin affinity chromatography (two times), anion-exchange chromatography and hydrophobic interaction chromatography. The purified factor, rat serum growth factor (R-SGF), was shown as a single band by SDS-gel electrophoresis and its molecular weight was estimated to be 32 kDa. The molecular weight was confirmed by gel filtration chromatography of the purified sample. The DNA synthesis-stimulating activity of R-SGF was lost by treating with protease, mercaptoethanol, acid and heating. R-SGF stimulated DNA synthesis of rat lung fibroblasts only when they were immortalized by going through crisis, whereas it had no effect on normal (mortal) cells tested before the crisis. This biological property of the factor was different from that of well-known heparin-binding growth factors, such as platelet-derived growth factor, acidic fibroblast growth factor and basic fibroblast growth factor, which stimulated DNA synthesis on both the mortal and immortal cells. These results indicate that this factor is a novel growth factor.
- 社団法人日本動物学会の論文
- 1993-12-15
著者
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KONDO Hiroshi
Department of Civil Engineering, School of Engineering
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Noumura Tetsuo
Department Of Biology Tokyo Metropolitan Institute Of Gerontology
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Noumura Tetsuo
Department Of Regulation Biology Saitama University
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Yonezawa Yumiko
Department Of Experimental Biology Tokyo Metropolitan Institute Of Gerontology
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Hirai Reiko
Section Of Tumor Cell Biology Tokyo Metropolotan Institute Of Medical Science
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Kondo Hiroshi
Department Of Agricultural Chemistry College Of Agriculture And Veterinary Medicine Nihon University
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