Immunohistochemical Localization of Epidermal Growth Factor in the Developing Rat Gonads

概要

論文の詳細を見る
Epidermal growth factor (EGF) is known to have various endocrine, paracrine and autocrine roles in adult mammalian tissues. In order to clarify the participation of EGF in rat gonadal differentiation, immunohistochemical localization of EGF as chronologically studied in perinatal rat gonads. Sprague-Dawley rat gonads from gestational day (GD) 13 to postnatal day (PD) 21 were fixed in acetic acid-free Bouin's solution and stained with a polyclonal antibody against mouse EGF by using avidin-biotin complex technique. Immunohistochemical reactivity was positive in almost all cell types in the prenatal male gonads. Male germ and Leydig /interstitial cells showed a positive reactivity from GD 15 to 21. Slight and moderate staining were seen in the Sertoli/ supporting cells from GD 13 to 21. After birth, positive expression was not seen in any types of cells in male gonads except for germ cells on PD 21. On the other hand, in prenatal female gonads positive signs were found in the interstitial cells from GD 14 to 21 and in the granulosa cells on GD 21. During the postnatal period from PD 5 to 21, the granulosa and theca cells were slightly positive and the interstitial cells moderately positive. Wolffian ducts in males and Mullerian ducts in females were stained during the prenatal period. These results indicate that EGF shows stage-specific patterns of expression in the developing rat gonadal cells and may be involved in the rat gonadal development and diffferentiation.
社団法人日本動物学会の論文
1994-02-15

Immunohistochemical Localization of Epidermal Growth Factor in the Developing Rat Gonads

スポンサーリンク

概要

著者

関連論文

スポンサーリンク