Apoptosis-Inducing Effects of Sterols from the Dried Powder of Cultured Mycelium of Cordyceps sinensis
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概要
- 論文の詳細を見る
The methanolic extract and its ethyl acetate-soluble fraction from the dried powder of cultured Cordyceps sinensis mycelium showed cytotoxic effects on promyelocytic leukemia HL-60 cells. Sitosterol (1), 5α,8α-epidioxy-22E-ergosta-6,22-dien-3β-ol (2), 5α,8α-epidioxy-22E-ergosta-6,9(11),22-trien-3β-ol (3), 5α,6α-epoxy-5α-ergosta-7,22-dien-3β-ol (4), and ergosterol (5) were isolated from the ethyl acetate fraction. Among the isolated compounds, 2-4 with a peroxide ring or an epoxide ring showed substantial cytotoxic activity with IC_<50> values of 7.3-7.8μg/ml, but 1 and 5 showed only moderate activity. Furthermore, apoptosis of HL-60 cells was observed 24h after treatment with 2-4 (10,20μg/ml) using the TdT-mediated dUTP nick-end labeling method, and their apoptosis-inducing activities are suggested to be dependent on the activation of caspases-3/7. To the best of our knowledge, this is the first report of the isolation of sterol constituents (1-5) from cultured C. sinensis mycelium.
- 2009-04-01
著者
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NAKAMURA Seikou
Kyoto Pharmaceutical University
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MATSUDA Hisashi
Kyoto Pharmaceutical University
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YOSHIKAWA Masayuki
Kyoto Pharmaceutical University
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吉川 雅之
Kyoto Pharmaceutical Univ.
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OKAZAKI Yoshie
Kyoto Pharmaceutical University
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山崎 幹夫
千葉大学薬学部
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Hiwatashi Kazuyuki
秋田県農林水産技術センター総合食品研究所
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Hiwatashi Kazuyuki
東北大学 農学研究科栄養学
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松田 秀秋
近畿大学 薬学部
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山崎 幹夫
千葉大学薬学部医薬品素材学講座
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Miwa H
Second Department Of Internal Medicine Mie University School Of Medicine
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Miyashita Hiroyuki
三重大学 医学部内科学第2
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AKAKI Junji
R & D Company, Kobayashi Pharmaceutical Co., Ltd.
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KOJIMA Hiroaki
R & D Company, Kobayashi Pharmaceutical Co., Ltd.
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TAMESADA Makoto
R & D Company, Kobayashi Pharmaceutical Co., Ltd.
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Mitani H
Second Department Of Internal Medicine Mie University School Of Medicine
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Mitani Hidetsugu
Division Of Hematology And Oncology Suzuka General Hospital
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Mitani Hidetsugu
三重大学 第2内科
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Yoshikawa M
Program In Tissue Engineering Department Of Parasitology Nara Medical University
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Miwa Hiroshi
三重大学 第2内科
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Yamazaki Masatoshi
Faculty Of Pharmaceutical Sciences Teikyo University Sagamiko
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Akaki Junji
R & D Company Kobayashi Pharmaceutical Co. Ltd.
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山田 美紀子
Faculty Of Pharmaceutical Sciences Chiba University
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Matsuda Hajime
Nihon Shokuhin Kako Co. Ltd.
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Yamazaki M
School Of Pharmaceutical Sciences Kinki University
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Matsuda Hideaki
Laboratory Of Natural Drug Resources Faculty Of Pharmacy Kinki University
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前田 初男
大阪大学大学院薬学研究科分子薬科学専攻
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前田 初男
大阪大学大学院薬学研究科
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Maeda H
Faculty Of Pharmaceutical Sciences Osaka University
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Maeda Hatsuo
Faculty Of Pharmaceutical Sciences Osaka University
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Kojima Hiroaki
R & D Company Kobayashi Pharmaceutical Co. Ltd.
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Tamesada Makoto
R & D Company Kobayashi Pharmaceutical Co. Ltd.
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Maeda Haruka
The Institute Of Physical And Chemical Research(riken)
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Hori K
Kao Co. Ltd. Tochigi Jpn
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Hori Kazuyuki
The Central Research Laboratory Hitachi Ltd.
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松田 久司
Kyoto Pharmaceutical Univ.
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Kojima Hiroaki
R&d Co. Kobayashi Pharmaceutical Co. Ltd.
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Kojima Hiroaki
Kobayashi Pharmaceutical Co. Ltd. R&d Company
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Nakamura Seikou
Department Of Applied Biology Kyoto Institute Of Technology
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Nakamura Seikou
Department of Applied Biology, Kyoto Institute of Technology
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MATSUDA HISASHI
Kyoto College of Pharmacy
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