Proteoglycans Regulate the Chemotaxis of Dendritic Cells Derived from Human Peripheral Blood Monocytes
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概要
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Dendritic cells (DCs) are a type of antigen-presenting cell which play an essential role in the immune system. The transition from immature DC (iDCs) to mature DCs (mDCs) requires appropriate maturation stimuli, such as pro-inflammatory cytokines or pathogen-derived components. Proteoglycans (PGs), which are composed of core proteins and the glycosaminoglycans that bind to them, are one of the main components of the extracellular matrix around pathogens such as bacteria. This study investigated the effects of PG extracted from the nasal septum cartilage of whale (W-PG) on the maturation of DCs derived from human peripheral blood monocytes. iDCs were prepared from human monocytes using granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). The iDCs were stimulated by W-PG alone. In another type of experiment, the iDCs were stimulated by MIX (tumor necrosis factor-α (TNF-α), IL-1β, IL-6 and prostaglandin E2 (PGE2)) or a combination of MIX plus W-PG. The stimulation of W-PG alone did not induce the phenotypic maturation from iDCs. However, W-PG promoted the up-regulation of chemokine receptor CCR7-surface expression and the chemotactic responsiveness to CCR7 ligand macrophage inflammatory protein-3β on MIX-stimulated mDCs although W-PG did not influence matrix metalloproteinase-9 activity which is an important factor in DC migration through the extracellular matrix. The findings that W-PG can selectively regulate the chemotactic activity of DCs in vitro under inflammatory conditions therefore indicate that the interaction of PGs with immune cells including DCs plays an important role in the immune response under the milieu of innate immunity.
著者
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TAKAHASHI Kenji
Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences
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KASHIWAKURA Ikuo
Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences
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Yoshino Hironori
Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Gra
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Monzen Satoru
Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Gra
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Takahashi Kenji
Dep. Of Radiological Life Sciences Hirosaki Univ. Graduate School Of Health Sciences
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Takahashi K
School Of Life Sciences Tokyo Univ. Of Pharmacy And Life Sciences
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Takahashi Kayoko
Central Research Lnstitute Meiji Milk Products Co. Ltd.
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Takahashi Kenji
Department Of Orthopaedics Graduate School Of Medical Science Kyoto Prefectural University Of Medici
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Takahashi K
Tokyo Univ. Agriculture And Technol. Tokyo Jpn
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Yoshino Hironori
Department Of Physics And Electronics Faculty Of Engineering Osaka Prefecture University
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Kashiwakura Ikuo
Department Of Radiological Life Sciences Division Of Medical Life Sciences Hirosaki University
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Takahashi Kenji
Department Of Biophysics And Biochemistry Graduate School Of Science The University Of Tokyo
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Takahashi Kenji
Department Of Atomic Science And Nuclear Engineering Faculty Of Engineering Hokkaido University
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Takahashi Kenji
Laboratory Of Molecular Biochemistry School Of Life Science Tokyo University Of Pharmacy And Life Sc
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Takahashi Kenji
Department Of Applied Biological Chemistry Graduate School Of Agriculture Kinki University
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KASHIWAKURA Ikuo
Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences
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TAKAHASHI Kenji
Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences
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Yoshino Hironori
Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences
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Monzen Satoru
Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences
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