Induction of Apoptosis through the Activation of SAPK/JNK Followed by the Expression of Death Receptor Fas in X-irradiated Cells

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概要

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• A post-irradiation treatment of the human leukemia cell line MOLT-4 with the anitoxidant Trolox attenuated capase-3 dependent apoptosis. The increase in the p53 expression and SAPK/JNK activation after X irradiation was also inhibited by a Trolox treatment, but the expression of BCL-2 and BAX, which would occur downstream from p53, was not changed. Studies on the effects of the intracellular calcium chelator BAPTA-AM on the induction of apoptosis and the activation of SAPK/JNK and capase-3 proved that the chelation of calcium merely delayed the onset of radiation-induced apoptosis and the activation of SAPK/JNK and caspase-3. When the effects of the protein synthesis inhibitor cycloheximde on the apoptotic signaling pathways, including the activation of caspase family proteins and SAPK/JNK, were investigated, the expression of death receptor Fas through SAPK/JNK activation was found to be required for radiation-induced apoptosis. Finally, the relationship between the amounts of DNA dsb and induction of apoptosis was examined by irradiating BrdU-incorporated cells. An increase in DNA dsb caused by BrdU was found, but the induction of apoptosis was not enhanced. From these data, we could get no positive evidence for DNA as a target of X-rays and p53 as an indispensable factor to induced apoptosis in X-irradiated MOLT-4 cells.

• 日本放射線影響学会の論文

著者

• Takahashi Kenji

  Dep. Of Radiological Life Sciences Hirosaki Univ. Graduate School Of Health Sciences

• Takahashi K

  School Of Life Sciences Tokyo Univ. Of Pharmacy And Life Sciences

• Takahashi Kayoko

  Central Research Lnstitute Meiji Milk Products Co. Ltd.

• KUWABARA MIKINORI

  Laboratory of Radiation Biology, Graduate School of Veterinary Medicine

• Takahashi K

  Tokyo Univ. Agriculture And Technol. Tokyo Jpn

• INANAMI Osamu

  Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of

• TAKAHASHI Kenji

  Division of Material Science, Graduate School of Natural Science and Technology, Kanazawa University

• Kuwabara Mikinori

  Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine

• Kuwabara Mikinori

  Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V

• Inanami Osamu

  Laboratory Of Radiation Biology Department Of Environmental Veterinary Medical Sciences Graduate Sch

• Inanami Osamu

  Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine Hokkaido University

• Takahashi Ken-ichi

  Department Of Industrial Chemistry Faculty Of Engineering Chiba Institute Of Technology

• Kuwabara Mikinori

  Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.

• Takahashi Kyoko

  Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'

• Kageyama T

  Kyoto Univ. Aichi Jpn

• Takahashi Kenji

  School Of Life Sciences Tokyo University Of Pharmacy And Life Sciences

• Takahashi Kenji

  Division Of Material Science Graduate School Of Natural Science And Technology Kanazawa University

• Takahashi Kenji

  Laboratory Of Molecular Biochemistry School Of Life Science Tokyo University Of Pharmacy And Life Sc

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