Effects of Ceramide Inhibition on Radiation-induced Apoptosis in Human Leukemia MOLT-4 Cells
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概要
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In the present study, using inhibitors of ceramide synthase (fumonisin B1), ketosphinganine synthetase (L-cycloserine), acid sphingomyelinase (D609 and desipramine) and neutral sphingomyelinase (GW4869), the role of ceramide in X-ray-induced apoptosis was investigated in MOLT-4 cells. The diacylglycerol kinase (DGK) assay showed that the intracellular concentration of ceramide increased time-dependently after X irradiation of cells, and this radiation-induced accumulation of ceramide did not occur prior to the appearance of apoptotic cells. Treatment with D609 significantly inhibited radiation-induced apoptosis, but did not inhibit the increase of intracellular ceramide. Treatment with desipramine or GW4869 prevented neither radiation-induced apoptosis nor the induced increase of ceramide. On the other hand, fumonisin B1 and L-cycloserine had no effect on the radiation-induced induction of apoptosis, in spite of significant inhibition of the radiation-induced ceramide. From these results, it was suggested that the increase of the intracellular concentration of ceramide was not essential for radiation-induced apoptosis in MOLT-4 cells.
- 日本放射線影響学会の論文
- 2006-03-31
著者
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浅沼 武敏
北海道大学大学院獣医学研究科環境獣医科学講座放射線学教室
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INANAMI Osamu
Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of
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Kuwabara Mikinori
弘前大学 保健学研究科放射線生命科学
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Kuwabara Mikinori
Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine
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Asanuma Taketoshi
Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V
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Asanuma Taketoshi
Laboratory Of Radiation Biology Department Of Environmental Veterinary Medicine Graduate School Of V
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Asanuma Taketoshi
Depertment Of Radiation Biology Faculty Of Veterinary Medicine Hokkaido University
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Inanami Osamu
Laboratory Of Radiation Biology Department Of Environmental Veterinary Medical Sciences Graduate Sch
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Inanami Osamu
Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine Hokkaido University
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TAKAHASHI Eriko
Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of
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KUWABARA Mikinori
Labs. Radiat. Biol.
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Kuwabara M
Hokkaido Univ. Sapporo Jpn
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Inanami Osamu
Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.
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Kuwabara Mikinori
Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.
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Takahashi Eriko
Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V
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Takahashi Eriko
Laboratory Of Pathobiology Department Of Veterinary Clinical Sciences School Of Veterinary Medicine
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