Conformational change in full-length mouse prion: a site-directed spin-labeling study.

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概要

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• The structure of the mouse prion (moPrP) was studied using site-directed spin-labeling electron spin resonance (SDSL-ESR). Since a previous NMR study by Hornemanna et al., [Hornemanna, Korthb, Oeschb, Rieka, Widera, Wüthricha, Glockshubera, Recombinant full-length murine prion protein, mPrP (23–231): purification and spectroscopic characterization, FEBS Lett. 413 (1997) 277–281] has indicated that N96, D143, and T189 in moPrP are localized in a Cu2+ binding region, Helix1 and Helix2, respectively, three recombinant moPrP mutations (N96C, D143C, and T189C) were expressed in an Escherichia coli system, and then refolded by dialysis under low pH and purified by reverse-phase HPLC. By using the preparation, we succeeded in preserving a target cystein residue without alteration of the α-helix structure of moPrP and were able to apply SDSL-ESR with a methane thiosulfonate spin label to the full-length prion protein. The rotational correlation times (τ) of 1.1, 3.3, and 4.8 ns were evaluated from the X-band ESR spectra at pH 7.4 and 20 °C for N96R1, D143R1, and T189R1, respectively. τ reflects the fact that the Cu2+ binding region is more flexible than Helix1 or Helix2. ESR spectra recorded at various temperatures revealed two phases together with a transition point at around 20 °C in D143R1 and T189R1, but not in N96R1. With the variation of pH from 4.0 to 7.8, ESR spectra of T189R1 at 20 °C showed a gradual increase of τ from 2.9 to 4.8 ns. On the other hand, the pH-dependent conformational changes in N96R1 and D143R1 were negligible. These results indicated that T189 located in Helix2 possessed a structure sensitive to physiological pH changes; simultaneously, N96 in the Cu2+ binding region and D143 in Helix1 were conserved.

• Elsevier Inc.の論文
• 2005-09-30

著者

• Kuwabara Mikinori

  弘前大学 保健学研究科放射線生命科学

• Kuwabara Mikinori

  Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine

• Inanami Osamu

  Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine Hokkaido University

• KUWABARA Mikinori

  Labs. Radiat. Biol.

• Kuwabara M

  Hokkaido Univ. Sapporo Jpn

• Hiraoka W

  Department Of Physics School Of Science And Technology Meiji University

• Kuwabara Mikinori

  Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.

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