Redox regulation in radiation-induced cytochrome c release from mitochondria of human lung carcinoma A549 cells.

スポンサーリンク

概要

• 論文の詳細を見る

• Mitochondria in mammalian cells were well-known to play an important role in the intrinsic pathway of genotoxic-agent-induced apoptosis by releasing cytochrome c into cytosol and to be a major source of reactive oxygen species (ROS). The aim of this study is to examine whether mitochondrial ROS involved in radiation-induced apoptotic signaling in A549 cells. The post-irradiation-treatment of N-acetyl-L-cystein (NAC) inhibited cytochrome c release from mitochondria but did not affect expression level of Bcl-2, Bcl-XL and Bax, suggesting late production of ROS triggered cytochrome c release. The experiments using DCFDA (a classical ROS fluorescence probe) and MitoAR (a novel mitochondrial ROS probe) demonstrated that intracellular and mitochondrial ROS were enhanced 6 h after X irradiation. Furthermore, the O2^[-.] production ability of mitochondria isolated from A549 cells was evaluated by ESR spectroscopy combined with a spin-trapping reagent (CYPMPO). When isolated mitochondria were incubated with NADH, succinate and CYPMPO, the ESR spectrum due to CYPMPO-OOH was detected. This NADH/succinate-dependent O2^[-.] production from mitochondria of irradiated cells was significantly increased in comparison with that of unirradiated cells. These results indicated that ionizing radiation enhanced O2^[-.] production from mitochondria to trigger cytochrome c release in A549 cells.

• Elsevierの論文
• 2009-05-08

著者

• 平山 暁

  筑波大学腎臓内科

• 平山 暁

  筑波大学臨床医学系腎臓内科

• 平山 暁

  筑波大学 大学院人間総合科学研究科

• Hirayama Aki

  筑波技術大学保健科学部附属東西医学統合医療センター

• Hirayama Aki

  Center For Integrative Medicine Tsukuba University Of Technology

• Hirayama Aki

  筑波大学 人間総合科学研究科

• Hirayama Aki

  Department Of Internal Medicine Institute Of Clinical Medicine University Of Tsukuba

• 平山 暁

  筑波大学 臨床医 腎臓内科

• Kon Yasuhiro

  Lab. Of Anat. Grad. Sch. Vet. Med. Hokkaido Univ.

• Kuwabara Mikinori

  弘前大学 保健学研究科放射線生命科学

• Kuwabara Mikinori

  Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine

• Inanami Osamu

  弘前大学 保健学研究科放射線生命科学

• Inanami Osamu

  Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine Hokkaido University

• KUWABARA Mikinori

  Labs. Radiat. Biol.

• Kuwabara M

  Hokkaido Univ. Sapporo Jpn

• Yasui Hironobu

  Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V

• Inanami Osamu

  Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.

• Kuwabara Mikinori

  Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.

• Yasui Hironobu

  Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V

関連論文

• A novel copper(II) coordination at His186 in full-length murine prion protein
• Effects of Overexpression and Antisense RNA Expression of Orf17, a MutT-Type Enzyme
• X Irradiation Combined with TNF α-related Apoptosis-inducing Ligand (TRAIL) Reduces Hypoxic Regions of Human Gastric Adenocarcinoma Xenografts in SCID Mice
• Enhancement of Cell Death by TNF α-related Apoptosis-inducing Ligand (TRAIL) in Human Lung Carcinoma A549 Cells Exposed to X Rays under Hypoxia
• 73)コレステロール塞栓症に対し,ステロイドセミパルス及び多量内服維持投与が奏功した一例(第202回日本循環器学会関東甲信越地方会)
• SF-083-3 肝虚血再灌流障害におけるElectron paramagnetic resonance imaging (EPRI)による臓器還元能評価(第107回日本外科学会定期学術集会)
• 79 腎細胞癌免疫療法中に急速進行性糸球体腎炎(RPGN)が出現し二重膜濾過血漿交換療法(DFPP)単独療法が奏功した1例
• 01 白血球吸着療法施行中の抗酸化動態
• 抗カルジオリピン抗体単独陽性を示した膜性増殖性糸球体腎炎の1例
• 220.オクタコサノール摂取が持久的トレーニング後の抗酸化能力及び酸化ストレスの変動に及ぼす影響 : SATプロジェクト175(栄養・消化,一般口演,第60回 日本体力医学会大会)
• ESRで評価した骨格筋活性酸素消去能力の加齢変化 : SATプロジェクト161(加齢・性差, 第59回日本体力医学会大会)
• 収縮特性の異なる筋から分画したミトコンドリアにおける活性酸素消去活性の定量 : ESRの応用(運動器, 第59回日本体力医学会大会)
• 473. 中高齢者における日常身体活動量が抗酸化能力および酸化ストレスに及ぼす影響 : SATプロジェクト117(加齢・性差)
• 309.日常活動量の違いがラット骨格筋のO_2^・-消去活性に及ぼす影響 : ESRによる検討・SATプロジェクト77(トレーニング)
• 307.日常活動量の違いがラット骨格筋のHO・消去活性に及ぼす影響 : ESRによる検討・SATプロジェクト78(トレーニング)
• 61.異なる骨格筋における活性酸素消去活性の比較 : 電子スピン共鳴法の応用の可能性・SATプロジェクト53(【運動器】)
• A BOLD-fMRI study of cerebral activation induced by injection of algesic chemical substances into the anesthetized rat forepaw
• Conformational change in full-length mouse prion: a site-directed spin-labeling study.
• Heterogeneity of Prognosis in Adult IgA Nephropathy, Especially with Mild Proteinuria or Mild Histological Features
• In Vivo Imaging of Renal Redox Status during Azelnidipine Treatment
• EXPRESSION OF TOXIC EFFECTS OF REACTIVE OXYGEN INTERMEDIATES IN CULTURED MAMMALIAN CELLS
• L-Arginine Administration Reverses Anemia Associated with Renal Disease
• Individual Differences in the Radiosensitivity of Hematopoietic Progenitor Cells Detected in Steady-State Human Peripheral Blood
• The Effects of (-)-Epigallocatechin-3-Gallate on the Proliferation and Differentiation of Human Megakaryocytic Progenitor Cells
• Activation of SAPK and increase of intracellular calcium during hydrogen peroxide-induced apoptosis in Chinese hamster lung fibroblast V79 cells
• 3P-016 マウスプリオンタンパク質ミュータントの線維形成(蛋白質-構造,第47回日本生物物理学会年会)
• 1P-015 変異マウスプリオンのpH依存性構造変化(蛋白質・構造(1),第46回日本生物物理学会年会)
• Redox regulation in radiation-induced cytochrome c release from mitochondria of human lung carcinoma A549 cells.
• Hepatocyte-Specific Deletion of Heme Oxygenase-1 Disrupts Redox Homeostasis in Basal and Oxidative Environments
• Effect of different dialyzers on defensins during hemodialysis
• Effects of BAPTA-AM and Forskolin on Apoptosis and Cytochrome C Release in Photosensitized Chinese Hamster V79 Cells
• Mechanisms of protective effects of PBN on X-ray-induced apoptosis in MOLT-4 cells
• H_2O_2-Induced Activation of SAPK/JNK Regulated by Phosphatidylinositol 3-Kinase in Chinese Hamster V79 Cells
• Radiation-chemical Properties of the Hypoxic Cell Radiosensitizer Doranidazole (PR-350)
• Sensitization of Radiation-induced Apoptosis of HL-60 Cells by Etanidazole
• Piperidine-sensitive base damage induced by γ-irradiation of frozen aqueous solution of double-stranded oligodeoxynucleotides containing 8-oxoguanine UDA
• Instability of familial spongiform encephalopathy-related prion mutants
• Lipid raft disruption prevents apoptosis induced by 2-chloro-2'-deoxyadenosine (Cladribine) in leukemia cell lines.
• 2P085 Phosphorylation-induced conformational change of p47^ : CW ESR and Four-Pulse DEER study by a site-directed spin labeling (SDSL)(30. Protein function (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Effects of Ceramide Inhibition on Radiation-induced Apoptosis in Human Leukemia MOLT-4 Cells
• Enhanced Induction of Apoptosis by Combined Treatment of Human Carcinoma Cells with X Rays and Death Receptor Agonists
• 268 A Novel Anticancer Ribonucleoside, TAS106 Radiosensitizes Apoptosis through Survivin Down-regulation and G2/M Checkpoint Abrogation in MKN45 Cells(Bases of radiotherapy, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
• 239 Inhibition of Cdc2 kinase activity by Purvalanol A sensitizes X rays-induced apoptosis in gastric tumor cells(Apoptosis related, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
• 154 Effects of hypoxia and doranidazole on X-ray-induced apoptotic signaling pathway(Radio-protection and sensitization, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
• Mechanisms of induction of apoptosis by co-treatment of X-rays with ECyd in Chinese Hamster V79 Cells
• Co-treatment of X-rays with ECyd induces Apoptosis through Down Regulation of Survivin in Gastric Tumor Cell Line MKN45 Cells
• Apoptosis signal transduction pathway derived from Golgi apparatus injury in light-illuminated HeLa cells in the presence of photosensitizer.
• Apoptotic Signaling and Redox Regulation in Bovine Endothelial Cells exposed to H_2O_2
• OH-induced free radicals in PolyA/PolyU : ESR and spin-trapping study
• Induction of Apoptosis through the Activation of SAPK/JNK Followed by the Expression of Death Receptor Fas in X-irradiated Cells
• CYTOTOXIC EFFECT OF ACYCLOVIR ON CULTURED MAMMALIAN CELLS TO WHICH HERPESVIRUS THYMIDINE KINASE GENE WAS INTRODUCED
• Analysis of T-cell receptor usage in myeloperoxidase-antineutrophil cytoplasmic antibody-associated renal vasculitis
• Radiation-induced apoptosis of tumor cells is facilitated by inhibition of the interaction between Survivin and Smac/DIABLO
• Identification of pH-sensitive regions in the mouse prion by the cysteine-scanning spin-labeling ESR technique.
• Regulation of cell survival and death signals induced by oxidative stress
• Treatment combining X-irradiation and a ribonucleoside anticancer drug, TAS106, effectively suppresses the growth of tumor cells transplanted in mice.
• Purvalanol A enhances cell killing by inhibiting up-regulation of CDC2 kinase activity in tumor cells irradiated with high doses of X rays.
• X irradiation induces the proapoptotic state independent of the loss of clonogenic ability in Chinese hamster V79 cells.
• Magnetic resonance imaging of hepatocellular carcinoma in Long-Evans Cinnamon rats under a magnetic field of 7.05 T
• Enhancement of X-Ray-Induced Cell Death in Chinese Hamster V79 Cells by ECyd
• 78 Existence of Megakaryocytic Progenitor Cells with Different Radiosensitivity Included in Normal Human Peripheral Blood(Radiation sensitivities in cells & tissues, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
• Synergistic Action of TPO plus IL-3 on the Proliferation and Differentiation of X-irradiated Megakaryocytic Progenitor Cells from Human Peripheral Blood
• Proliferation-Promoting and Radioprotective Activities of Amifostine on the Clonal Growth of MegakaryocyticProgenitor Cells
• Radiation Sensitivity of CD34+ Megakaryocyte Colony-Forming Cells from Human Umbilical Cord Blood and Peripheral Blood and the Effects of Cytokines on Megakaryopoiesis In Vitro.
• Spin-trapping detection of superoxides in polymorphonuclear leukocytes stimulated with serum-opsonized zymosan
• Induction of neurite outgrowth by α-phenyl-N-tert-butylnitrone through nitric oxide release and Ras-ERK pathway in PC12 cells
• A nucleoside anticancer drug, 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine (TAS106), sensitizes cells to radiation by suppressing BRCA2 expression
• A novel anticancer ribonucleoside, 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine, enhances radiation-induced cell death in tumor cells.
• Activation of C-Kit by Stem Cell Factor Induces Radioresistance to Apoptosis through ERK-dependent Expression of Survivin in HL60 Cells
• Apoptosis Induction in Gastric Adenocarcinoma MKN45 Cells Exposed to X-Rays in the Presence of 1-(3-CEthynyl-
• Attenuation of caspase-3-dependent apoptosis in X-irradiated MOLT-A cells by the post-treatment with Trolox, an inhibitor of lipid peroxdation
• Apoptosis and Signal Transduction in X-irradiated MOLT-4 Cells
• Activation of SAPK and ICE-like protease during X-ray-induced apoptosis in human leukemia cell line MOLT-4
• 8-Aminoadenosine Enhances Radiation-induced Cell Death in Human Lung Carcinoma A549 Cells
• Radiation-Induced Activation of NADH Oxidase in MOLT-4 Cells
• Involvement of Caspase-8 and -9 on X-Ray-induced Apoptosis in MOLT-4 Cells
• 8-Aminoadenosine Enhances Radiation-induced Cell Death in Human Lung Carcinoma A549 Cells
• Ionizing radiation induces mitochondrial reactive oxygen species production accompanied by upregulation of mitochondrial electron transport chain function and mitochondrial content under control of the cell cycle checkpoint
• Vincristine enhances amoeboid-like motility via GEF-H1/RhoA/ROCK/Myosin light chain signaling in MKN45 cells

もっと見る 閉じる

スポンサーリンク