Enhancement of Cell Death by TNF α-related Apoptosis-inducing Ligand (TRAIL) in Human Lung Carcinoma A549 Cells Exposed to X Rays under Hypoxia
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概要
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Our previous study showed that ionizing radiation induced the expression of death receptor DR5 on the cell surface in tumor cell lines and that the death receptor of the TNF alpha-related apoptosis-inducing ligand TRAIL enhanced the apoptotic pathway (Hamasu et al., (2005) Journal of Radiation Research, 46:103-110). The present experiments were performed to examine whether treatment with TRAIL enhanced the cell killing in tumor cells exposed to ionizing radiation under hypoxia, since the presence of radioresistant cells in hypoxic regions of solid tumors is a serious problem in radiation therapy for tumors. When human lung carcinoma A549 cells were irradiated under normoxia and hypoxia, respectively, radiation-induced enhancement of expression of DR5 was observed under both conditions. Incubation in the presence of TRAIL enhanced the caspase-dependent and chymotrypsin-like-protease-dependent apoptotic cell death in A549 cells exposed to X rays. Furthermore, it was shown that treatment with TRAIL enhanced apoptotic cell death and loss of clonogenic ability in A549 cells exposed to X rays not only under normoxia but also under hypoxia, suggesting that combination treatment with TRAIL and X irradiation is effective for hypoxic tumor cells.
- 2007-11-15
著者
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Kubota Nobuo
Pola Chemical Industries Inc.
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TSUJITANI MICHIHIKO
Pola Chemical Industries, Inc.
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Kubota Nobuo
Department Of Radiological Sciences. Ibaraki Prefectural University Of Health Sciences
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KUWABARA MIKINORI
Laboratory of Radiation Biology, Graduate School of Veterinary Medicine
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INANAMI Osamu
Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of
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Kuwabara Mikinori
弘前大学 保健学研究科放射線生命科学
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Kuwabara Mikinori
Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine
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Kuwabara Mikinori
Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V
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Inanami Osamu
弘前大学 保健学研究科放射線生命科学
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Inanami Osamu
Laboratory Of Radiation Biology Department Of Environmental Veterinary Medical Sciences Graduate Sch
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Inanami Osamu
Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine Hokkaido University
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TAKAHASHI Momoko
Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of
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YASUI Hironobu
Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of
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OGURA Aki
Laboratory of Radiation Biology, Department of Environmental Veterinary Sciences, Graduate School of
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KUWABARA Mikinori
Labs. Radiat. Biol.
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Kuwabara M
Hokkaido Univ. Sapporo Jpn
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Ogura Aki
Laboratory Of Radiation Biology Department Of Environment Veterinary Sciences School Of Veterinary M
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Yasui Hironobu
Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V
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Inanami Osamu
Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.
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Kuwabara Mikinori
Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.
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Takahashi Momoko
Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V
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Tsujitani Michihiko
Pola Chemical Industries
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