CYTOTOXIC EFFECT OF ACYCLOVIR ON CULTURED MAMMALIAN CELLS TO WHICH HERPESVIRUS THYMIDINE KINASE GENE WAS INTRODUCED
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概要
- 論文の詳細を見る
The cytotoxic effects of acyclovir, which is a purine nucleoside analogue and is known as an antibiotic substance, were examined on three lines of rat skin fibroblast FR cells ; normal FR cells, FRtk^- cells which are deficient in the activity of thymidine kinase (tk) and FRtk^-HSVtk^+ cells which were prepared by introducing herpes simplex virus' tk gene to FRtk^- cells. When FRtk^-HSVtk^+ cells growing exponentially were incubated in the presence of acyclovir for 4 h, the surviving fractions of the cells decreased in a concertration-dependent manner. Whereas, decrease of the surviving fractions was almost indiscernible in both FR cells and FRtk^- cells at the whole ranges of drug-concentrations tested. These results indicate that acyclovir is phosphorylated by the herpes simplex virus' tk and becomes toxic to FRtk^-HSVtk^+ cells. This also means that FRtk^-HSVtk^+ cells are useful for the investigation of the biological activity of nucleoside analogues.
- 北海道大学の論文
- 1989-11-10
著者
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HIRAOKA Wakako
Department of Physics, School of Science and Technology, Meiji University
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Tanabe Kiyoshi
富山医科薬科大学 放射線基礎医
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Tanabe Kiyoshi
Department Of Radiation Biology Faculty Of Veterinary Medicine Hokkaido University
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SATO Fumiaki
Department of Surgical Neurology Jichi Medical School
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Sato F
Japan Racing Assoc. Utsunomia
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Sato Fumiaki
Faculty Of Veterinary Medicine Hokkaido Univ.
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Narita Takahiro
Department Of Epizootiology Faculty Of Veterinary Medicine Hokkaido University
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Narita Takahiro
Department Of Applied Electronics Engineering Hokkaido Inst
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Kuwabara Mikinori
Department Of Physics Faculty Of Science Hokkaido University
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Kuwabara M
Hokkaido Univ. Sapporo Jpn
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Sato F
Equine Research Institute
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NIIKURA Masahiro
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University
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Hiraoka Wakako
Department Of Physics School Of Science And Technology Meiji University
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Hiraoka W
Department Of Physics School Of Science And Technology Meiji University
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Sato F
Hokkaido Univ. Sapporo
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Niikura Masahiro
Special Pathogens Laboratory Department Of Virology 1 National Institute Of Infectious Diseases
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Sato Fumiaki
Department Of Computational Intelligence And Systems Science Tokyo Institute Of Technology
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Tanabe Kiyoshi
Department Of Physics Kagoshima University
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Hiraoka Wakako
Department Of Physics Graduate School Of Science And Technology Meiji University
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Sato Fumiaki
Department of Clinical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University
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TANABE Kiyoshi
Dept. Radiol. Sci., Toyama Medical and Pharmaceutical Univ.
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TANABE Kiyoshi
Department of Physics, Kagoshima University
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KUWABARA Mikinori
Department of Environmental Veterinary Science, Graduate School of Veterinary Medicine, Hokkaido University
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Tanabe Kiyoshi
Department of Chemistry, Faculty of Science, The University of Tokyo
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