CYTOCHEMICAL STUDY OF THE ELECTRON MICROSCOPICAL LOCALIZATION OF K^+-DEPENDENT p-NITROPHENYLPHOSPHATASE ACTIVITY ON CHOROIDAL EPENDYMAL EPITHELIUM IN NORMAL RAT BRAIN : COMPARING WITH THE ACTIVITY OF Mg^<2+>-ATPASE AND ALKALINE PHOSPHATASE
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概要
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To determine the ultracytochemical localization of Na^+, K^+-ATPase activity on normal rat choroidal epithelium, K^+ dependent-p-nitrophenylphosphatase activity was studied by using the one-step lead citrate method devised by Mayahara et al. (1978). An electron dense reaction product was found to be abundant inside the microvilli and positively along the outside of the basal plasmalemmas of the choroidal epithelium. No reaction product was seen on the apical and lateral ones. In the cytoplasm, the electron dense precipitates were also observed in the endoplasmic reticulum and nuclear membrane. Ouabain (10^<-3>-10^<-4>M) effectively inhibited the activities in the microvilli and basal plasmalemmas. Alkaline phosphatase reaction stronger than that of Mg^<2+>-ATPase was observed mainly on the outside of the basal plasmalemmas, but the activities of these two enzymes were not found in the microvilli.
- 日本組織細胞化学会の論文
著者
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SAITO Takuma
Department of Anatomy, Jichi Medical School
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Saito Takuma
Departments Of Anatomy Jichi Medical School
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MASUZAWA Toshio
Department of Surgical Neurology Jichi Medical School
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SATO Fumiaki
Department of Surgical Neurology Jichi Medical School
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Masuzawa Toshio
Department Of Neurosurgery Jichi Medical School
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Masuzawa Toshio
Departments Of Surgical Neurology Jichi Medical School
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Sato Fumiaki
Departments Of Surgical Neurology Jichi Medical School
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Saito Takuma
Department Of Anatomy Jichi Medica1 School
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Masuzawa Toshio
Department Of Surgical Neurology Jichi Medica1 School
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Sato Fumiaki
Department Of Computational Intelligence And Systems Science Tokyo Institute Of Technology
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Saito Takuma
Department Of Anatomy And Ophthalmology Jichi Medical School
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Sato Fumiaki
Department of Clinical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University
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