Involvement of Reactive Oxygen Species in Abnormal Tropoelastin Deposition Induced by UVA-Photosensitizers
スポンサーリンク
概要
- 論文の詳細を見る
Chronically, sun-exposed human skin is characterized by dermal connective tissue damage with the accumulation of abnormal elastic fibers. However, little is known about the relationship between accumulation of abnormal elastic fibers and photodamaged skin. In the present study, we investigated the involvement of reactive oxygen species (ROS) in photoaged skin including abnormal accumulation of tropoelastin (TE) induced by ultraviolet A (UVA)-irradiation using an in vitro model of elastic fiber formation. Our data showed that the morphological appearances of TE deposition was observed following the addition of recombinant TE immediately after treatment with UVA-irradiation by immunofluorescence staining and semi-quantitative assay. Our data also revealed that treatment with hypoxanthine-xanthine oxidase, which generates superoxide radicals, stimulated TE deposition. Furthermore, we confirmed that abnormal TE deposition induced by UVA-irradiation was inhibited by treatment with Cu/Zn superoxide dismutase, a superoxide radical scavenger. Therefore, the data obtained suggest that superoxide radical is a candidate for inducing morphological changes and increasing TE deposition induced by UVA-irradiation in human skin fibroblast cells. The present study would be helpful for developing a prophylactic agent for photoaged skin.
著者
-
Wachi Hiroshi
Department Of Clinical Chemistry Hoshi University School Of Pharmacy And Pharmaceutical Sciences
-
Wachi Hiroshi
Department Of Clinical Chemistry Hoshi College Of Pharmacy
-
SATO Fumiaki
Department of Surgical Neurology Jichi Medical School
-
NONAKA Risa
Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences
-
Onoue Satoshi
Research & Development Division, KOSÉ Corporation
-
Koide Chiharu
Research & Development Division, KOSÉ Corporation
-
Hayashi Akinobu
Research & Development Division, KOSÉ Corporation
-
Sato Fumiaki
Department Of Computational Intelligence And Systems Science Tokyo Institute Of Technology
-
Sato Fumiaki
Department of Clinical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University
-
Nonaka Risa
Department of Clinical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University
-
Koide Chiharu
Research & Development Division, KOSÉ Corporation
-
Onoue Satoshi
Research & Development Division, KOSÉ Corporation
-
Wachi Hiroshi
Department of Clinical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University
-
Hayashi Akinobu
Research & Development Division, KOSÉ Corporation
関連論文
- Immunohistochemical Study of Na^+, K^+-ATPase in the Choroid Plexus
- Matrix metalloproteinase(MMP)-9 gene expression is coordinately modulated by TPA responsive element and KRE-M9 element - A novel mechanism of MMP-9 gene expression implies a role for keratinization
- Miochondrial DNA Deletions and Aging induced by Low Dose Rate of Cs γ-ray in Mice
- Characterization of the Molecular Interaction between Tropoelastin and DANCE/Fibulin-5
- CYTOCHEMICAL STUDY OF THE ELECTRON MICROSCOPICAL LOCALIZATION OF K^+-DEPENDENT p-NITROPHENYLPHOSPHATASE ACTIVITY ON CHOROIDAL EPENDYMAL EPITHELIUM IN NORMAL RAT BRAIN : COMPARING WITH THE ACTIVITY OF Mg^-ATPASE AND ALKALINE PHOSPHATASE
- The Cytochemical Study of the Electron Microscopical Localization of K^+ dependent-p-Nitrophenylphosphatase Activity on Choroidal Ependymal Epithelium in Normal Rat Brain. : Comparing with the Activity of Mg^ ATPase and Alkaline Phosphatase.
- Proteolytic Release of Latent Transforming Growth Factor-β (TGF-β) Binding Protein-1 (LTBP-1) Fragment in Wound Healing
- Characteristic expression of connective tissue components and matrix metalloproteinases (MMPs) during the development of pressure ulcerations
- Upregulation of rhoA mRNA in Bronchial Smooth Muscle of Antigen-induced Airway Hyperresponsive Rats
- Characterization of an in vitro Model of Calcification in Retinal Pigmented Epithelial Cells
- Endothelin-1 Down-Regulates Expression of Tropoelastin and Lysyl Oxidase mRNA in Cultured Chick Aortic Smooth Muscle Cells
- Involvement of Reactive Oxygen Species in Abnormal Tropoelastin Deposition Induced by UVA-Photosensitizers
- Squalene as a Target Molecule in Skin Hyperpigmentation Caused by Singlet Oxygen
- CYTOTOXIC EFFECT OF ACYCLOVIR ON CULTURED MAMMALIAN CELLS TO WHICH HERPESVIRUS THYMIDINE KINASE GENE WAS INTRODUCED
- MICRONUCLEUS TEST IN MICE FED ON AN IRRADIATED DIET
- THE EFFECTS OF IONIZING RADIATION ON PROMOTER-DEPENDENT RNA SYNTHESIS
- Concentration of Metallothionein in Mice Livers after a Small Dose of Irradiation
- Parasellar Chondrosarcoma : -Case Report-
- Intracerebral Hemorrhage Immediately Following Surgical Treatment of Chronic Subdural Hematoma : Report of Two Cases
- S-A-7 S-A-7. Biochemistry, Immunonogy and Tissue Culture Studies in Brain Tumors (Symposium A: Pathology and Biochemistry of Brain Tumors)
- Experimental and Clinical Studies of Radiosensitizers in Brain Tumors, with Special Reference to BUdR-Antimetabolite Continuous Regional Infusion-Radiation Therapy (BAR therapy)
- cDNA microarray analysis of esophageal cancer : discoveries and prospects
- 7-Ketocholesterol, a Major Oxysterol, Promotes Pi-Induced Vascular Calcification in Cultured Smooth Muscle Cells
- Treatment with Vitamin K2 Combined with Bisphosphonates Synergistically Inhibits Calcification in Cultured Smooth Muscle Cells
- Biochemical Analysis of Elastic Fiber Formation with a Frameshift-Mutated Tropoelastin (fmTE) at the C-Terminus of Tropoelastin
- Tropoelastin Inhibits Vascular Calcification via 67-kDa Elastin Binding Protein in Cultured Bovine Aortic Smooth Muscle Cells
- Role of tropoelastin exon 16 in elastic fiber assembly
- Exon 16 deficient human tropoelastin stimulate the MG63 cell proliferation
- Nitric Oxide Stimulates Elastin Expression in Chick Aortic Smooth Muscle Cells
- Effects of Monensin on Tropoelastin Metabolism in Vascular Smooth Muscle Cells: Monensin Causes Intracellular Degradation of Accumulated Tropoelastin
- Modulation of Elastin Expression by Heparin Is Dependent on the Growth Condition of Vascular Smooth Muscle Cells: Up-Regulation of Elastin Expression by Heparin in the Proliferating Cells Is Mediated by the Inhibition of Protein Kinase C Activity
- Ultrastructural Immunocytochemistry of Tetanus Toxin Binding Sites to Spinal Ganglia of Chick Embryo in Tissue Culture
- Cytochemical Study of Various Phosphatase : Activities on the Capillary Walls of Rat Cerebral Cortex
- Ultrastractural histochemistry of Ca^-ATPase in rat hippocampus
- Enzyme histochemistry of ornithine carbamoyl transferase (OCT) and carbamoyl phosphate phosphatase (CPP) in rat choroid plexus
- Cytochemical localization of various ATPase activities on rat sciatic nerve
- Enzyme Cytochemistry of Choroid Plexuses of Experimental Hydrocephalic Rats.
- Cytochemical Study of Alkaline Phosphatase Activity in Microvessels of Rat Cerebral Cortex
- Choroid Plexus Papillomas and Their Fine Structure
- Ultrastructural Localization of Carbonic Anhydrase(CAHase) Activities in the Rat Choroid Plexus(chp).
- The Localization of Glucose-6-phosphatase(G6Pase) and Thiamine Pyrophosphatase(TPPase) Activities in the Rat Choroid Plexus.
- Alkaline Phosphatase in the Cultured Glial Cells of the Neural Retina and Optic Lobe of Chick Embryos
- Alkaline phosphatase and Acetylcholin-esterase in Cultured Neural Retinal Cells
- Reaction Synthesis of Ti_3 SiC_2 from Mixture of Elemental Powders
- Post-Translational Regulation of Type I Collagen Synthesis by Heparin in Vascular Smooth Muscle Cells
- Effects of Bidens pilosa L. var. radiata SCHERFF on experimental gastric lesion
- A Versatile Approach to 2-Substituted 3-Trifluoromethyl-1,3-diols Based on the Reaction of Trifluoroacetaldehyde Ethyl Hemiacetal with Enamines Derived from Aldehydes
- Development of Agent-Based In-Store Simulator to Analyze Pedestrian Behaviors
- A COMPARISON OF RADIOIMMUNOASSAY WITH SPECTROPHOTOMETRY FOR THE DETERMINATION OF PLASMA DIPHENYLHYDANTOIN CONCENTRATIONS
- Atherosclerosis and Matrix Dystrophy
- 74. Syphon Body Tumor
- 10. Tissue culture of glioma
- MicroRNA-141 confers resistance to cisplatin-induced apoptosis by targeting YAP1 in human esophageal squamous cell carcinoma
- Role of Elastic Fibers on Cardiovascular Disease
- 20. Complications of the Ventriculo-Atrial Shunt Operation. (Proceedings of the XXVth Annual Meeting of the Japan Neurosurgical Society)
- Effects of Monensln on Tropoelastin Metabolism in Vascular Smooth Muscle Cells: Monensin Causes Intracellular Degradation of Accumulated Tropoelastin.