Endothelin-1 Down-Regulates Expression of Tropoelastin and Lysyl Oxidase mRNA in Cultured Chick Aortic Smooth Muscle Cells
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概要
- 論文の詳細を見る
Endothelin-1(ET-1) is known as a potent stimulator of cell proliferation and as a vasoconstrictor. It is believed that ET-1 contributes to the development of arterial diseases such as atherosclerosis. In this study, we demonstrated the expression of tropoelastin and lysyl oxidase(LO) on gene levels as induced by ET-1 in cultured smooth muscle cells(SMCs). ET-1 stimulated cell proliferation in a dose-dependent manner, and the level of this proliferation increased about 1.3-fold at 100 nM of ET-1. ET-1 suppressed the tropoelastin protein synthesis in a dose-dependent and time-dependent manner. In addition, ET-1 dose-dependently suppressed the tropoelastin and LO mRNA expression. The tropoelastin and LO mRNA levels decreased to about half and 4/5, respectively, at 100 nM of ET-1. The inhibition of elastin synthesis was completely prevented by BQ123, an endothelin receptor A(ET_A) antagonist. These results indicate that ET-1 can modulate the tropoelastin and LO mRNA expression via an ET_A receptor in cultured SMC and that the regulator for elastin expression may play an important role in elastogenesis and SMC proliferation during the development of atherosclerosis.
- 公益社団法人日本薬学会の論文
- 2001-12-01
著者
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Sakamoto Yoshimitsu
星薬科大学 臨床化学
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Wachi Hiroshi
Faculty Of Technokogy Tokyo University Of Agriculture And Technology
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Wachi Hiroshi
Department Of Clinical Chemistry Hoshi University School Of Pharmacy And Pharmaceutical Sciences
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Wachi Hiroshi
Department Of Clinical Chemistry Hoshi College Of Pharmacy
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TAJIMA Shingo
Department of Dermatology, National Defense Medical Collage
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Wachi Hiroshi
星薬科大学 臨床化学教室
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SEYAMA Yoshiyuki
Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences
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SUGITANI Hideki
Department of Clinical Chemistry, School of Pharmacy, Hoshi University
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Seyama Yoshiyuki
Department Of Clinical Chemistry Hoshi College Of Pharmacy
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Tajima Shingo
Department Of Dermatology Keio University School Of Medicine
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Sakai Hiroyasu
Department Of Pharmacology School Of Pharmacy Hoshi University
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Sakai H
Department Of Pharmaceutical Physiology Graduate School Of Medicine And Pharmaceutical Sciences Univ
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Tajima Shingo
Department Of Dermatology Keio University
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Sugitani Hideki
Department Of Clinical Chemistry Hoshi University School Of Pharmacy And Pharmaceutical Sciences
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SEYAMA YOSHIYUKI
Department of Biochemistry, Tokyo College of Pharmacy
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TAJIMA Shingo
Department of Dermatiology, Keio University Schooh of Medicine
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