Nitric Oxide Stimulates Elastin Expression in Chick Aortic Smooth Muscle Cells
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概要
- 論文の詳細を見る
Nitric oxide (NO), an endothelium-dependent relaxing factor, regulates relaxation, proliferation, and migration of smooth muscle cells (SMCs) and most likely attenuates developing vascular disease such as atherosclerosis. We investigated whether or not NO is associated with regulation of aortic elasticity. S-Nitrosoglutathione (GSNO), a NO donor, stimulated tropoelastin synthesis in cultured SMCs durin both the quiescent and proliferating phases. The stimulation of tropoelastin synthesis was dose-dependent within 1-100 nM. Maximum stimulation was detected by treatment with 100nM GSNO for 24h. 8-Bromoguanosine 3', 5'-cyclic monophosphate (8-Br-cGMP), an exogenous cyclic GMP analog, also upregulated tropoelastin synthesis. Tropoelastine and lysyl oxidase mRNA expression, as assessed by Northern blot analysis, was also stimulated by GSNO. Administration of KT5823, a cyclic GMP-dependent protein kinase inhibitor, inhibited the GSNO-induced tropoelastin synthesis. These results indicate that the stimulatory effects of GSNO are due to cyclic GMP dependent protein kinase (PKG) activation by NO. In conclusion, NO seems to enhance aortic elasticity via tropoelastin and lysyl oxidase upregulation.
- 公益社団法人日本薬学会の論文
- 2001-05-01
著者
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Sakamoto Yoshimitsu
星薬科大学 臨床化学
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Wachi Hiroshi
Faculty Of Technokogy Tokyo University Of Agriculture And Technology
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Wachi Hiroshi
Department Of Clinical Chemistry Hoshi University School Of Pharmacy And Pharmaceutical Sciences
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Wachi Hiroshi
Department Of Clinical Chemistry Hoshi College Of Pharmacy
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TAJIMA Shingo
Department of Dermatology, National Defense Medical Collage
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Wachi Hiroshi
星薬科大学 臨床化学教室
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SEYAMA Yoshiyuki
Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences
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SUGITANI Hideki
Department of Clinical Chemistry, School of Pharmacy, Hoshi University
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Seyama Yoshiyuki
Department Of Clinical Chemistry Hoshi College Of Pharmacy
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Tajima Shingo
Department Of Dermatology Keio University School Of Medicine
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Sakai Hiroyasu
Department Of Pharmacology School Of Pharmacy Hoshi University
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Sakai H
Department Of Pharmaceutical Physiology Graduate School Of Medicine And Pharmaceutical Sciences Univ
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Tajima Shingo
Department Of Dermatology Keio University
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Sugitani Hideki
Department Of Clinical Chemistry Hoshi University School Of Pharmacy And Pharmaceutical Sciences
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SEYAMA YOSHIYUKI
Department of Biochemistry, Tokyo College of Pharmacy
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TAJIMA Shingo
Department of Dermatiology, Keio University Schooh of Medicine
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