TNF-α augments the expression of RhoA in the rat bronchus
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概要
- 論文の詳細を見る
While nonspecific airway hyperresponsiveness (AHR) is a central feature of allergic bronchial asthma, the mechanism underlying the development of AHR is not clearly understood. We have previously demonstrated in vitro hyperresponsiveness of bronchial smooth muscle to acetylcholine (Ach) in rats that were actively sensitized and repeatedly challenged with aerosolized antigen. It has also been demonstrated that the Ach-induced, RhoA-mediated Ca^<2+> sensitization is markedly augmented concomitantly with an increased expression and activation of RhoA protein in the bronchial smooth muscle of the antigen-treated rats. In the present study, we have investigated whether TNF-α, a proinflammatory cytokine which is involved in bronchial asthma, causes upregulation of RhoA mRNA and protein in the rat bronchus. Treatment of rat bronchial smooth muscle preparations with TNF-α (300 ng/ml for 24 hr) significantly shifted the concentration-response curve to Ach upwards, but did not alter the response to high K^+, when compared to that of control tissues. Levels of RhoA mRNA and protein in the TNF-α-treated bronchus were significantly greater than those in the control group. In conclusion, it is suggested that the augmentation of the Ach-induced contractile response evoked by TNF-α might be mediated by an upregulation of RhoA in rat bronchial smooth muscle.
- 日本平滑筋学会の論文
著者
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CHIBA Yoshihiko
Department of Pharmacology, School of Pharmacy, Hoshi University
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MISAWA Miwa
Department of Pharmacology, School of Pharmacy, Hoshi University
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SAKAI Hiroyasu
Department of Internal Medicine, Gifu University Graduate School of Medicine
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OTOGOTO Shinobu
Department of Pharmacology, School of Pharmacy, Hoshi University
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ABE Kazuho
Department of Pharmacology, School of Pharmacy, Hoshi University
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Chiba Yoshihiko
Department Of Pharmacology School Of Pharmacy Hoshi University
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Abe Kazuho
Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences The University Of Tokyo
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Misawa Miwa
Department Of Applied Pharmacology Hoshi University
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Sakai Hiroyasu
Department Of Pharmacology School Of Pharmacy Hoshi University
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Sakai H
Department Of Pharmaceutical Physiology Graduate School Of Medicine And Pharmaceutical Sciences Univ
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Otogoto Shinobu
Department Of Pharmacology School Of Pharmacy Hoshi University
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Chiba Yoshihiko
Department Of Biology School Of Pharmacy Hoshi University
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