DRUG EFFECTS ON ELECTRICAL ACTIVITIES OF THE CANINE TRACHEAL CILIATED CELLS
スポンサーリンク
概要
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Effects of drugs on the tracheal ciliated cell of dogs were studied with reference to relationship between intracellular electrical activity and ciliary movement. The mucosal specimen of tracheal membranous wall of animals was mounted in a chamber containing Hanks solution at 36° The bioelectrical activity was recorded by the microelectrode method, and the changes in ciliary motility was estimated by measuring the mucus transport rate. At a depth of 5-25 μ from the mucous surface, a negative potential of a magnitude of -28.6±1.1 mV, was obtained. This resting potential had oscillating potential changes, with the amplitude of 200.4±20.9 μV, and the frequency of 13.8±1.2 Hz. Elevated K ion of the external medium of up to 40 mM caused a slight membrane depolarization without accompanying any spike potential. The depolarization appeared with increases in amplitude and frequency of the oscillatory potential and in ciliary motility, while a stronger depolarization over 15 mV with 60-120 mM K ion decreased the amplitude, frequency and motility. Acetylcholine and adrenaline (10^<-6>-10^<-2>M) caused a cilioexcitation, and increased amplitude and frequency of the oscillatory potential. On the other hand, 5-hydroxytryptamine, dibucaine and lidocaine caused both cilioinhibition and decreases in amplitude and frequency. These findings suggest that the oscillations are the electrical activity which is closely related to the mechanical activity in the beating cells ; the amplitude of oscillatory potential is probably correlating to ciliary beating force and the frequency correlating to beat frequency. Intracellular electrical activities of the ciliated cell are, thus, useful indices for elucidating properties of ciliated cells and in studying a mechanism of drug action on ciliary motility.
- 公益社団法人日本薬学会の論文
著者
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TAKEDA Hiroshi
Department of Medical Information Science, Osaka University Graduate School of Medicine
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MISAWA Miwa
Department of Pharmacology, School of Pharmacy, Hoshi University
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YANAURA Saizo
Department of Pharmacology, School of Pharmacy, Hoshi University
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IMAMURA Naoto
Department of Thoracic Surgery, Nishi-Kobe Medical Center
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Takeda Hiroshi
Department Of Earth And Planetary Science The University Of Tokyo
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HOSOKAWA Tomokazu
Department of Pharmacology, School of Pharmacy, Hoshi University
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Hosokawa Tomokazu
Department Of Pharmacology Hoshi College Of Pharmacy
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Misawa Miwa
Department Of Applied Pharmacology Hoshi University
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Imamura Naoto
Department Of Thoracic Surgery Nishi-kobe Medical Center
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Misawa Miwa
Department Of Pharmacology Hoshi College Of Pharmacy
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Yanaura Saizo
Department Of Pharmacology Hoshi College Of Pharmacy
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Yanaura Saizo
Department Of Applied Pharmacology School Of Pharmacy Hoshi University
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Takeda Hiroshi
Department Of Pharmacology School Of Pharmacy Hoshi University
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Takeda Hiroshi
Department Of Abdominal Surgery Tenri Hospital
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Hukuhara T
Jikei Univ. School Of Medicine Tokyo
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Hosokawa Tomokazu
Department of Medicinal Plant Science, Hoshi University
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IMAMURA Naoto
Department of Pharmacology, Hoshi College of Pharmacy
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YANAURA Saizo
Department of Applied Pharmacology, School of Pharmacy, Hoshi University:Research Institute of Nippon Chemiphar, Co., Ltd.
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