Identification and Characterization of Rat RhoA Gene Promoter

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概要

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• RhoA upregulation has been suggested in bronchial smooth muscles (BSMs) of asthmatic rats. Here, we cloned/characterized the 5′-promoter region of the rat rhoA. A transcription-initiation site was identified at 66-bp upstream of the reference sequence, GenBank-BC061732. Luciferase assay using interleukin-13 (IL-13)–stimulated cells revealed a significant promoter activity at 238- to 166-bp upstream of the transcription-initiation site, which contains a signal transducer and activation of transcription (STAT) 6–binding region. The IL-13–induced increase in luciferase activity was inhibited by a STAT6 inhibitor, AS1517499, or a Janus kinases (JAKs) inhibitor, JAK Inhibitor-I, but not by tyrphostin-AG490, WHI-P131, or tyrphostin-AG9 (selective JAK2, JAK3, and Tyk2 inhibitors, respectively). Thus, rat BSM rhoA expression may have causal relation to the IL-13–JAK1–STAT6 signaling.

著者

• CHIBA Yoshihiko

  Department of Pharmacology, School of Pharmacy, Hoshi University

• MISAWA Miwa

  Department of Pharmacology, School of Pharmacy, Hoshi University

• Goto Kumiko

  Department of Pharmacology, School of Pharmacy, Hoshi University, Japan

• Matsusue Kimihiko

  Faculty of Pharmaceutical Science, Fukuoka University, Japan

• Kimura Shioko

  Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, USA

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