Acetylcholine-Induced Translocation of RhoA in Freshly Isolated Single Smooth Muscle Cells of Rat Bronchi
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概要
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By using immunofluorostaining and confocal laser microscopy, acetylcholine-induced translocation of RhoA was visualized in freshly isolated bronchial smooth muscle cells of the rat. The cellular distribution of RhoA at rest was observed uniformly in the cytosolic space with no staining in the nucleus, whereas acetylcholine stimulation induced a relocalization of RhoA to the cell periphery. From the results of line scans and surface plots, the peripheral to cytosolic ratio of RhoA was significantly increased by acetylcholine stimulation. Thus, the present study clearly demonstrated an acetylcholine-induced translocation of RhoA to the plasma membrane in single bronchial smooth muscle cells of the rat.
著者
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SAKAI Hiroyasu
Department of Internal Medicine, Gifu University Graduate School of Medicine
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OKU Teruaki
Department of Biochemistry, Hoshi University
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ITOH Saotomo
Department of Biochemistry, Hoshi University
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Misawa Miwa
Department Of Applied Pharmacology Hoshi University
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Tsuji Tsutomu
Department Of Legal Medicine Wakayama Medical University
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Chiba Yoshihiko
Department Of Biology School Of Pharmacy Hoshi University
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UCHIDA Tetsuro
Department of Chemistry, Nagaoka University of Technology
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Tsuji Tsutomu
Department of Microbiology, School of Pharmacy, Hoshi University
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Oku Teruaki
Department of Microbiology, School of Pharmacy, Hoshi University
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Itoh Saotomo
Department of Microbiology, School of Pharmacy, Hoshi University
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