Exclusion of Actin-Binding Protein p57/Coronin-1 from Bacteria-Containing Phagosomes in Macrophages Infected with Legionella(Microbiology)

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概要

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• Legionella pneumophila, the causative agent of Legionnaires' disease, is a human pathogen that multiplies within alveolar macrophages. L. pneumophila establishes specialized phagosomes in which it evades the host defense through largely unknown mechanisms. Here we analyzed the role of an actin-binding protein, p57/coronin-1, a member of the coronin protein family, during Legionella infection. On fluorescence microscopy, p57/coronin-1 and F-actin were found to be co-localized at the sites on the plasma membrane where L. pneumophila adhered to U937 human macrophage-like cells. The localization of p57/coronin-1 at the sites of bacterial adherence was inhibited by treatment with cytochalasin D (an inhibitor of actin polymerization), suggesting that p57/coronin-1 is involved in the actin-dependent uptake of L. pneumophila into U937 cells. In addition, we showed that p57/coronin-1 was excluded from phagosomes containing live L. pneumophila throughout the infection, whereas transient accumulation of p57/coronin-1 was observed on phagosomes containing Texas-Red-labeled opsonized zymosan (TROpZ) or heat-killed L. pneumophila at an early stage of phagocytosis. The exclusion of p57/coronin-1 from phagosomes containing live another Legionella species Legionella gratiana at an early stage of infection was also observed. Taken together, these results suggest that the endocytic pathways of live Legionella species are distinct from general phagocytic pathways, which lead to lysosomal degradation.

• 公益社団法人日本薬学会の論文
• 2008-05-01

著者

• Imai Yasuyuki

  Laboratory of Microbiology and Immunology and the Global COE Program, University of Shizuoka School

• Tsuji Toshikazu

  岐阜薬科大学 薬理学

• Tsuji Toshikazu

  Department Of Pharmacology Gifu Pharmaceutical University

• TSUJI Tsutomu

  Department of Legal Medicine, Wakayama Medical University

• Daimon Takashi

  Laboratory Of Drug Evaluation And Informatics University Of Shizuoka School Of Pharmaceutical Scienc

• OKU Teruaki

  Department of Biochemistry, Hoshi University

• TOYOSHIMA Satoshi

  Pharmaceuticals and Medical Devices Evaluation Center National Institute of Health Sciences

• Oku Teruaki

  Department Of Microbiology Hoshi University School Of Pharmacy And Pharmaceutical Sciences

• Oku Teruaki

  Department Of Microbiology School Of Pharmacy Hoshi University

• ITOH Saotomo

  Department of Biochemistry, Hoshi University

• Fukui Takashi

  Laboratory Of Microbiology And Immunology And The Global Coe Program University Of Shizuoka School O

• Toyoshima S

  Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hoshi University

• Toyoshima S

  Pharmaceuticals And Medical Devices Agency

• HAYASHI Tsuyoshi

  Laboratory of Microbiology and Immunology and the Global COE Program, University of Shizuoka School

• MIYAKE Masaki

  Laboratory of Microbiology and Immunology and the Global COE Program, University of Shizuoka School

• SUGAYA Noriko

  Laboratory of Microbiology and Immunology and the Global COE Program, University of Shizuoka School

• Toyoshima Satoshi

  Pharmaceutical And Medical Devices Evaluation Center National Institute Of Health Science

• Oku T

  Amagasaki Chemical Industries Co.

• Sugaya Noriko

  Laboratory Of Microbiology And Immunology And The Global Coe Program University Of Shizuoka School O

• Imai Yasuyuki

  Laboratory Of Microbiology And Immunology And The Global Coe Program University Of Shizuoka School O

• Miyake Masaki

  Laboratory Of Microbiology And Immunology And The Global Coe Program University Of Shizuoka School O

• Tsuji T

  Department Of Microbiology Hoshi University School Of Pharmacy And Pharmaceutical Sciences

• Tsuji Tsutomu

  Department Of Legal Medicine Wakayama Medical University

• Imai Yasuyuki

  Laboratory Of Microbiology And Immunology And Coe Program In The 21st Century University Of Shizuoka

• Itoh Saotomo

  Department Of Microbiology Hoshi University School Of Pharmacy And Pharmaceutical Sciences

• Sakane T

  St. Marianna Univ. School Of Medicine Kanagawa Jpn

• Miyake Masaki

  Laboratory Of Microbiology And Immunology And The Global Coe Program School Of Pharmaceutical Scienc

• Hayashi Tsuyoshi

  Laboratory Of Microbiology And Immunology And The Global Coe Program University Of Shizuoka School O

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