Analysis of Differentiation Marker Proteins in Helper T Cell Subtypes, Th1 and Th2
スポンサーリンク
概要
- 論文の詳細を見る
Human helper T cells are divided into T helper(Th)1 and Th2 cells, which are known to have important roles in cell-mediated and humoral immunity, respectively. In the present study, we attempted to identify cell markers that can distinguish Th1 and Th2 cells using large-scale two-dimensional electrophoresis. Several proteins were found to be specifically and reproducibly expressed in each helper T cell subtype. Three proteins, which have molecular weights of 35000(spot 1), 40000(spot 2)and 49000(spot 3)with pIs of 7.0, 7.0 and 6.3, respectively, were found only in Th1 cells, and the other three proteins, which have molecular weights of 32500(spot 4), 38000(spot 5)and 44000(spot 6)with pIs of 6.5, 6.0 and 5.8, respectively, only in Th2 cells. Spots 1, 3 and 4 were found in both the membrane and the cytosol. Spots 2 and 5 were membrane proteins, while spot 6 was a cytosol protein. The molecular weight of spot 4 resembled that of ST2, which was reported to be a specific cell surface marker of Th2 cells. However, anti-ST2 antibody did not react with spot 4 at all. These results suggest that the proteins found in the present study are novel cell markers of Th1 and Th2 cells.
- 社団法人日本薬学会の論文
- 2001-06-01
著者
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Suzuki Akiko
Departments of Radiology, Yokohama City University, School of Medicine
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TOYOSHIMA Satoshi
Department of Pathology, Kitakyushu Municipal Medical Center
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Suzuki A
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hoshi University
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YAMASHITA Shozo
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hoshi University
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Kashiwakura Jun-ichi
Department of Biochemistry, Hoshi University
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Saitoh Kaori
Department of Biochemistry, Hoshi University
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Suzuki Akiko
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hoshi University
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SUZUKI Akio
Hoshi University School of Pharmacy and Pharmaceutical Sciences
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Kashiwakura Jun-ichi
Department Of Biochemistry Hoshi University
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Toyoshima S
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hoshi University
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Toyoshima S
Pharmaceuticals And Medical Devices Agency
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Toyoshima Satoshi
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hoshi University
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Saitoh Kaori
Department Of Biochemistry Hoshi University
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Yamashita S
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hoshi University:laboratory Of Racing
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Sakane T
St. Marianna Univ. School Of Medicine Kanagawa Jpn
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Suzuki Akiko
Department Of Anesthesiology University Of Hirosaki School Of Medicine
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