Carbonyl Reductase Activity Exhibited by Pig Testicular 20β-Hydroxysteroid Dehydrogenase
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概要
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The carbonyl reductase activity exhibited by pig testicular 20β-hydroxysteroid dehydrogenase (20β-HSD) was examined using a recombinant enzyme. Kinetic parameters were obtained for 48 carbonyl group-containing substrates, including aromatic aldehydes, aromatic ketones, cycloketones, quinones, aliphatic aldehydes and aliphatic ketones. 20β-HSD showed a high affinity towards quinones, such as 9,10-phenanthrenequinone, α-naphthoquinone and menadione (K_m values of 4,2 and 5 μM, respectively), and the substrate utilization efficiency (V_<max>/K_m) of the enzyme against these quinones was very high. Cyclohexanone and 2-methylcyclohexanone were also reduced with a high V_<max>/K_m value, but not cyclopentanone or 2-methylcyclopentanone. Various aromatic aldehydes and ketones including benzaldehyde- and acetophenone-derivatives were reduced by 20β-HSD. Especially, 4-nitrobenzaldehyde and 4-nitroacetophenone were reduced with high V_<max>/K_m values in related compounds. The enzyme also reduced the pyridine-derivatives, 2-, 3-, and 4-benzoylpyridine, with the V_<max>/K_m value for 2-benzoylpyridine being the highest. 20β-HSD reduced aliphatic aldehydes and aliphatic ketones, but was more effective on the former. The correlation between the structure of carbonyl compounds and their substrate V_<max>/K_m is discussed.
- 公益社団法人日本薬学会の論文
- 1997-11-15
著者
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TOYOSHIMA Satoshi
Department of Pathology, Kitakyushu Municipal Medical Center
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Nakajin Shizuo
Department of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences
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TAKASE Noriko
Department of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences
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Toyoshima Satoshi
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hoshi University
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TAMURA Fumihiro
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hoshi University
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Takase Noriko
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hoshi University
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Nakajin Shizuo
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hoshi University
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Tamura F
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hoshi University
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Tamura Fumihiro
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Hoshi University
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