Lipid raft disruption prevents apoptosis induced by 2-chloro-2'-deoxyadenosine (Cladribine) in leukemia cell lines.

スポンサーリンク

概要

• 論文の詳細を見る

• To clarify the role of lipid rafts in 2-chloro-2′-deoxyadenosine (2CdA; Cladribine)-induced apoptosis, the effects of disruption of lipid rafts by methyl-β-cyclodextrin (MβCD) and filipin on 2CdA-induced apoptosis were investigated in four human acute lymphoblastic leukemia (ALL) cell lines comprised of T cells (MOLT-4, Jurkat) and B cells (NALM, BALL-1). The disruption of lipid rafts significantly inhibited 2CdA-induced apoptosis, indicating the crucial role of lipid rafts in the induction of apoptosis in leukemia cells. These reagents significantly inhibited 2CdA-induced elevation of the intracellular calcium concentration ([Ca2+]i) in MOLT-4 cells, and 2CdA-induced apoptosis was partly inhibited by the Ca2+ chelators BAPTA-AM and EGTA, and the L-type Ca2+ channel blocker nifedipine. On the other hand, they had no effects on the cellular uptake of 2CdA. These results indicated that lipid rafts partly contributed to 2CdA-induced apoptosis by regulating Ca2+ influx via the plasma membrane.

• Elsevier Ltd.の論文

著者

• Kuwabara Mikinori

  弘前大学 保健学研究科放射線生命科学

• Kuwabara Mikinori

  Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine

• Inanami Osamu

  弘前大学 保健学研究科放射線生命科学

• Inanami Osamu

  Laboratory Of Radiation Biology Department Of Environmental Veterinary Medical Sciences Graduate Sch

• Inanami Osamu

  Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine Hokkaido University

• KUWABARA Mikinori

  Labs. Radiat. Biol.

• Kuwabara M

  Hokkaido Univ. Sapporo Jpn

• Inanami Osamu

  Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.

• Kuwabara Mikinori

  Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.

• Takahashi Eriko

  Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V

• Matsuda Akira

  Laboratory Of Medicinal Chemistry Faculty Of Pharmaceutical Sciences Hokkaido University

関連論文

• A novel copper(II) coordination at His186 in full-length murine prion protein
• Effects of Overexpression and Antisense RNA Expression of Orf17, a MutT-Type Enzyme
• X Irradiation Combined with TNF α-related Apoptosis-inducing Ligand (TRAIL) Reduces Hypoxic Regions of Human Gastric Adenocarcinoma Xenografts in SCID Mice
• Enhancement of Cell Death by TNF α-related Apoptosis-inducing Ligand (TRAIL) in Human Lung Carcinoma A549 Cells Exposed to X Rays under Hypoxia
• A BOLD-fMRI study of cerebral activation induced by injection of algesic chemical substances into the anesthetized rat forepaw
• Conformational change in full-length mouse prion: a site-directed spin-labeling study.
• The Antiproliferative Effect of Bovine Lactoferrin on Canine Mammary Gland Tumor Cells
• EXPRESSION OF TOXIC EFFECTS OF REACTIVE OXYGEN INTERMEDIATES IN CULTURED MAMMALIAN CELLS
• Individual Differences in the Radiosensitivity of Hematopoietic Progenitor Cells Detected in Steady-State Human Peripheral Blood
• The Effects of (-)-Epigallocatechin-3-Gallate on the Proliferation and Differentiation of Human Megakaryocytic Progenitor Cells
• Activation of SAPK and increase of intracellular calcium during hydrogen peroxide-induced apoptosis in Chinese hamster lung fibroblast V79 cells
• 3P-016 マウスプリオンタンパク質ミュータントの線維形成(蛋白質-構造,第47回日本生物物理学会年会)
• 1P-015 変異マウスプリオンのpH依存性構造変化(蛋白質・構造(1),第46回日本生物物理学会年会)
• Redox regulation in radiation-induced cytochrome c release from mitochondria of human lung carcinoma A549 cells.
• Effects of BAPTA-AM and Forskolin on Apoptosis and Cytochrome C Release in Photosensitized Chinese Hamster V79 Cells
• Mechanisms of protective effects of PBN on X-ray-induced apoptosis in MOLT-4 cells
• H_2O_2-Induced Activation of SAPK/JNK Regulated by Phosphatidylinositol 3-Kinase in Chinese Hamster V79 Cells
• Radiation-chemical Properties of the Hypoxic Cell Radiosensitizer Doranidazole (PR-350)
• Sensitization of Radiation-induced Apoptosis of HL-60 Cells by Etanidazole
• Piperidine-sensitive base damage induced by γ-irradiation of frozen aqueous solution of double-stranded oligodeoxynucleotides containing 8-oxoguanine UDA
• The Increased Formation of Alkali-Labile Sites by a Spin-Trapping Agent α-Phenyl-N-tert-Butylnitrone in γ-Irradiated Frozen Aqueous Solution of DNA
• Instability of familial spongiform encephalopathy-related prion mutants
• Lipid raft disruption prevents apoptosis induced by 2-chloro-2'-deoxyadenosine (Cladribine) in leukemia cell lines.
• 2P085 Phosphorylation-induced conformational change of p47^ : CW ESR and Four-Pulse DEER study by a site-directed spin labeling (SDSL)(30. Protein function (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Effects of Ceramide Inhibition on Radiation-induced Apoptosis in Human Leukemia MOLT-4 Cells
• Enhanced Induction of Apoptosis by Combined Treatment of Human Carcinoma Cells with X Rays and Death Receptor Agonists
• 268 A Novel Anticancer Ribonucleoside, TAS106 Radiosensitizes Apoptosis through Survivin Down-regulation and G2/M Checkpoint Abrogation in MKN45 Cells(Bases of radiotherapy, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
• 239 Inhibition of Cdc2 kinase activity by Purvalanol A sensitizes X rays-induced apoptosis in gastric tumor cells(Apoptosis related, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
• 154 Effects of hypoxia and doranidazole on X-ray-induced apoptotic signaling pathway(Radio-protection and sensitization, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
• Mechanisms of induction of apoptosis by co-treatment of X-rays with ECyd in Chinese Hamster V79 Cells
• Co-treatment of X-rays with ECyd induces Apoptosis through Down Regulation of Survivin in Gastric Tumor Cell Line MKN45 Cells
• Apoptosis signal transduction pathway derived from Golgi apparatus injury in light-illuminated HeLa cells in the presence of photosensitizer.
• Apoptotic Signaling and Redox Regulation in Bovine Endothelial Cells exposed to H_2O_2
• OH-induced free radicals in PolyA/PolyU : ESR and spin-trapping study
• Induction of Apoptosis through the Activation of SAPK/JNK Followed by the Expression of Death Receptor Fas in X-irradiated Cells
• CYTOTOXIC EFFECT OF ACYCLOVIR ON CULTURED MAMMALIAN CELLS TO WHICH HERPESVIRUS THYMIDINE KINASE GENE WAS INTRODUCED
• Radiation-induced apoptosis of tumor cells is facilitated by inhibition of the interaction between Survivin and Smac/DIABLO
• Identification of pH-sensitive regions in the mouse prion by the cysteine-scanning spin-labeling ESR technique.
• Regulation of cell survival and death signals induced by oxidative stress
• Treatment combining X-irradiation and a ribonucleoside anticancer drug, TAS106, effectively suppresses the growth of tumor cells transplanted in mice.
• Purvalanol A enhances cell killing by inhibiting up-regulation of CDC2 kinase activity in tumor cells irradiated with high doses of X rays.
• X irradiation induces the proapoptotic state independent of the loss of clonogenic ability in Chinese hamster V79 cells.
• Magnetic resonance imaging of hepatocellular carcinoma in Long-Evans Cinnamon rats under a magnetic field of 7.05 T
• Enhancement of X-Ray-Induced Cell Death in Chinese Hamster V79 Cells by ECyd
• 78 Existence of Megakaryocytic Progenitor Cells with Different Radiosensitivity Included in Normal Human Peripheral Blood(Radiation sensitivities in cells & tissues, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
• Synergistic Action of TPO plus IL-3 on the Proliferation and Differentiation of X-irradiated Megakaryocytic Progenitor Cells from Human Peripheral Blood
• Proliferation-Promoting and Radioprotective Activities of Amifostine on the Clonal Growth of MegakaryocyticProgenitor Cells
• Radiation Sensitivity of CD34+ Megakaryocyte Colony-Forming Cells from Human Umbilical Cord Blood and Peripheral Blood and the Effects of Cytokines on Megakaryopoiesis In Vitro.
• Spin-trapping detection of superoxides in polymorphonuclear leukocytes stimulated with serum-opsonized zymosan
• Induction of neurite outgrowth by α-phenyl-N-tert-butylnitrone through nitric oxide release and Ras-ERK pathway in PC12 cells
• A nucleoside anticancer drug, 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine (TAS106), sensitizes cells to radiation by suppressing BRCA2 expression
• A novel anticancer ribonucleoside, 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine, enhances radiation-induced cell death in tumor cells.
• Activation of C-Kit by Stem Cell Factor Induces Radioresistance to Apoptosis through ERK-dependent Expression of Survivin in HL60 Cells
• Apoptosis Induction in Gastric Adenocarcinoma MKN45 Cells Exposed to X-Rays in the Presence of 1-(3-CEthynyl-
• Attenuation of caspase-3-dependent apoptosis in X-irradiated MOLT-A cells by the post-treatment with Trolox, an inhibitor of lipid peroxdation
• Apoptosis and Signal Transduction in X-irradiated MOLT-4 Cells
• Activation of SAPK and ICE-like protease during X-ray-induced apoptosis in human leukemia cell line MOLT-4
• 8-Aminoadenosine Enhances Radiation-induced Cell Death in Human Lung Carcinoma A549 Cells
• Radiation-Induced Activation of NADH Oxidase in MOLT-4 Cells
• Involvement of Caspase-8 and -9 on X-Ray-induced Apoptosis in MOLT-4 Cells
• 8-Aminoadenosine Enhances Radiation-induced Cell Death in Human Lung Carcinoma A549 Cells
• Ionizing radiation induces mitochondrial reactive oxygen species production accompanied by upregulation of mitochondrial electron transport chain function and mitochondrial content under control of the cell cycle checkpoint
• Vincristine enhances amoeboid-like motility via GEF-H1/RhoA/ROCK/Myosin light chain signaling in MKN45 cells

もっと見る 閉じる

スポンサーリンク