Treatment combining X-irradiation and a ribonucleoside anticancer drug, TAS106, effectively suppresses the growth of tumor cells transplanted in mice.
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概要
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Purpose: To examine the in vivo antitumor efficacy of X-irradiation combined with administration of a ribonucleoside anticancer drug, 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine (TAS106, ECyd), to tumor cell–transplanted mice.Methods and Materials: Colon26 murine rectum adenocarcinoma cells and MKN45 human gastric adenocarcinoma cells were inoculated into the footpad in BALB/c mice and severe combined immunodeficient mice, respectively. They were treated with a relatively low dose of X-irradiation (2 Gy) and low amounts of TAS106 (0.1 mg/kg and 0.5 mg/kg). The tumor growth was monitored by measuring the tumor volume from Day 5 to Day 16 for Colon26 and from Day 7 to Day 20 for MKN45. Histologic analyses for proliferative and apoptotic cells in the tumors were performed using Ki-67 immunohistochemical and terminal deoxynucleotidyl transferase-mediated nick end labeling staining. The expression of survivin, a key molecule related to tumor survival, was assessed by quantitative polymerase chain reaction and immunohistochemical analysis.Results: When X-irradiation and TAS106 treatment were combined, significant inhibition of tumor growth was observed in both types of tumors compared with mice treated with X-irradiation or TAS106 alone. Marked inhibition of tumor growth was observed in half of the mice that received the combined treatment three times at 2-day intervals. Parallel to these phenomena, the suppression of survivin expression and appearance of Ki-67–negative and apoptotic cells were observed.Conclusions: X-irradiation and TAS106 effectively suppress tumor growth in mice. The inhibition of survivin expression by TAS106 is thought to mainly contribute to the suppression of the tumor growth.
- Elsevier Inc.の論文
- 2007-05-01
著者
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浅沼 武敏
北海道大学大学院獣医学研究科環境獣医科学講座放射線学教室
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Kon Yasuhiro
Lab. Of Anat. Grad. Sch. Vet. Med. Hokkaido Univ.
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Kuwabara Mikinori
弘前大学 保健学研究科放射線生命科学
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Kuwabara Mikinori
Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine
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Asanuma Taketoshi
Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V
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Asanuma Taketoshi
Laboratory Of Radiation Biology Department Of Environmental Veterinary Medicine Graduate School Of V
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Asanuma Taketoshi
Depertment Of Radiation Biology Faculty Of Veterinary Medicine Hokkaido University
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Inanami Osamu
弘前大学 保健学研究科放射線生命科学
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Inanami Osamu
Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine Hokkaido University
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KUWABARA Mikinori
Labs. Radiat. Biol.
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Kuwabara M
Hokkaido Univ. Sapporo Jpn
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Inanami Osamu
Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.
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Kuwabara Mikinori
Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.
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Matsuda Akira
Laboratory Of Medicinal Chemistry Faculty Of Pharmaceutical Sciences Hokkaido University
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Yasui Hironobu
Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V
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