Radiation-induced apoptosis of tumor cells is facilitated by inhibition of the interaction between Survivin and Smac/DIABLO
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概要
- 論文の詳細を見る
To investigate the mechanism of radioresistance of solid tumor cells, we created two expression vectors encoding Survivin mutants, T34A and D53A. When T34A and D53A were overexpressed in NIH3T3, A549 and HeLa cells, radiation-induced apoptosis was significantly enhanced. Furthermore, we examined the binding capability of Survivin with Smac/DIABLO in the cells that overexpressed these mutants. Coimmunoprecipitation analysis revealed that mutant form of Survivin, D53A and T34A could bind to Smac/DIABLO, but with much less affinity compared to the authentic form. These results suggest that radiation-induced apoptosis of tumor cells is increased by inhibition of the interaction between Survivin and Smac/DIABLO through overexpression of T34A and D53A.
- Elsevierの論文
- 2008-01-18
著者
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Kuwabara Mikinori
弘前大学 保健学研究科放射線生命科学
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Kuwabara Mikinori
Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine
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Inanami Osamu
弘前大学 保健学研究科放射線生命科学
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Inanami Osamu
Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine Hokkaido University
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KUWABARA Mikinori
Labs. Radiat. Biol.
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Kuwabara M
Hokkaido Univ. Sapporo Jpn
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Yasui Hironobu
Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V
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Inanami Osamu
Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.
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Kuwabara Mikinori
Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.
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Yasui Hironobu
Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V
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