Induction of neurite outgrowth by α-phenyl-N-tert-butylnitrone through nitric oxide release and Ras-ERK pathway in PC12 cells
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概要
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It has previously been suggested that the spin trap agent α-phenyl-N-tert-butylnitrone (PBN) induces neurite outgrowth through activation of the Ras-ERK pathway in PC12 cells. However, the chemical properties of PBN contributing to its biological function and the detailed mechanism for the activation of Ras by PBN remain unknown. This study demonstrates that the hydrophobic structure of PBN is related to the activation of Ras, by comparing with hydrophilic analogues of PBN. [14C]-labelled PBN was found to localize in the lipid fraction and activate Ras indirectly. On the other hand, neurite outgrowth by PBN was inhibited by a nitric oxide (NO) scavenger. Moreover, the neurite outgrowth induced by PBN and the NO donor NOR4 was inhibited by the dominant negative Ras or MAPK/ERK inhibitor. Taken together, these results suggest that PBN is incorporated into the plasma membrane and induces neurite outgrowth in PC12 cells by activating the Ras-ERK pathway through NO release
- Informa Healthcareの論文
著者
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浅沼 武敏
北海道大学大学院獣医学研究科環境獣医科学講座放射線学教室
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Kuwabara Mikinori
弘前大学 保健学研究科放射線生命科学
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Kuwabara Mikinori
Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine
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Asanuma Taketoshi
Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V
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Asanuma Taketoshi
Laboratory Of Radiation Biology Department Of Environmental Veterinary Medicine Graduate School Of V
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Asanuma Taketoshi
Depertment Of Radiation Biology Faculty Of Veterinary Medicine Hokkaido University
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Inanami Osamu
弘前大学 保健学研究科放射線生命科学
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Inanami Osamu
Laboratory Of Radiation Biology Graduate School Of Veterinary Medicine Hokkaido University
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KUWABARA Mikinori
Labs. Radiat. Biol.
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Kuwabara M
Hokkaido Univ. Sapporo Jpn
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Yasui Hironobu
Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V
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Inanami Osamu
Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.
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Kuwabara Mikinori
Lab. Radiat. Biol. Grad. Sch. Vet. Med. Hokkaido Univ.
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Yamamori Tohru
Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V
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Yasui Hironobu
Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V
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