Concentration-Dependent Modes of Cell Death in Chinese Hamster V79 Cells after Treatments with H_2O_2
スポンサーリンク
概要
- 論文の詳細を見る
Deterioration in the clonogenic ability of Chinese hamster V79 cells was observed after treatments with low concentrations of H_2O_2 (0.06-0.2 mM) for 1 h and subsequent incubation for 6-8 days, whereas no loss of ability of the cells to exclude trypan blue after treatments with H_2O_2 for 1 h and subsequent incubation for 6 h was observed in this concentration range. The loss of the dye-exclusion ability became observable when the cells were treated with concentrations greater than 0.9 mM H_2O_2 for 1 h and subsequently incubated for 6 h. Agarose gel electrophoresis of DNA extracted from cells treated with 1-5 mM H_2O_2 showed specific regular fragmentation of DNA, suggesting that apoptotic cell death was induced. The induction of apoptotic cell death was further confirmed by observing the protective effects of several modifiers, a protein synthesis inhibitor (cycloheximide), a Ca^<2+> -chelator (BAPTA-AM) and an antioxidative compound (PBN), against cell survival and DNA fragmentation. From these results, it was concluded that apoptotic cell death was induced by H_2O_2 in Chinese hamster cells at high concentrations, where their clonogenic ability completely disappeared.
- 日本放射線影響学会の論文
著者
-
Kuwabara Mikinori
Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V
-
Fuma Kaori
Laboratory Of Radiation Biology Department Of Environmental Veterinary Sciences Graduate School Of V
-
Hiraoka W
Department Of Physics School Of Science And Technology Meiji University
-
Hiraoka Wakako
Department Of Physics Graduate School Of Science And Technology Meiji University
関連論文
- A novel copper(II) coordination at His186 in full-length murine prion protein
- Enhancement of Cell Death by TNF α-related Apoptosis-inducing Ligand (TRAIL) in Human Lung Carcinoma A549 Cells Exposed to X Rays under Hypoxia
- Confirmation of enhanced expression of heme oxygenase-1 gene induced by ultrasound and its mechanism : analysis by cDNA microarray system, real-time quantitative PCR, and Western blotting
- Conformational change in full-length mouse prion: a site-directed spin-labeling study.
- 2P227 酸化ストレスによるアポトーシスにおけるO-結合型N-アセチルグルコサミン(O-GlcNAc)修飾の影響(細胞生物的課題(接着,運動,骨格,伝達,膜),第48回日本生物物理学会年会)
- 2P016 ヒトプリオンタンパク質におけるHis96とHis111のCu2+結合能力(蛋白質-構造,第48回日本生物物理学会年会)
- The Antiproliferative Effect of Bovine Lactoferrin on Canine Mammary Gland Tumor Cells
- Reactions between Hydroxyl-Radical-Induced 7,8-Dihydro-8-oxo-2'-deoxyguanosine Precursor and the Spin Trap α-Phenyl-N-tert-Butylnitrone
- Damage Induced by Hydroxyl Radicals Generated in the Hydration Layer of γ-Irradiated Frozen Aqueous Solution of DNA
- EXPRESSION OF TOXIC EFFECTS OF REACTIVE OXYGEN INTERMEDIATES IN CULTURED MAMMALIAN CELLS
- Sensitization of X-Irradiated Chinese Hamster V79 Cells by Derivatives of Pyrimidine Nucleosides
- Modification of the Repair of Potentially Lethal Damage in Plateau-Phase Chinese Hamster Cells by 2-Chlorodeoxyadenosine
- Individual Differences in the Radiosensitivity of Hematopoietic Progenitor Cells Detected in Steady-State Human Peripheral Blood
- The Effects of (-)-Epigallocatechin-3-Gallate on the Proliferation and Differentiation of Human Megakaryocytic Progenitor Cells
- Activation of SAPK and increase of intracellular calcium during hydrogen peroxide-induced apoptosis in Chinese hamster lung fibroblast V79 cells
- 3P-016 マウスプリオンタンパク質ミュータントの線維形成(蛋白質-構造,第47回日本生物物理学会年会)
- 1P-015 変異マウスプリオンのpH依存性構造変化(蛋白質・構造(1),第46回日本生物物理学会年会)
- Effects of BAPTA-AM and Forskolin on Apoptosis and Cytochrome C Release in Photosensitized Chinese Hamster V79 Cells
- Radiation-chemical Properties of the Hypoxic Cell Radiosensitizer Doranidazole (PR-350)
- Sensitization of Radiation-induced Apoptosis of HL-60 Cells by Etanidazole
- The Increased Formation of Alkali-Labile Sites by a Spin-Trapping Agent α-Phenyl-N-tert-Butylnitrone in γ-Irradiated Frozen Aqueous Solution of DNA
- Instability of familial spongiform encephalopathy-related prion mutants
- 2P085 Phosphorylation-induced conformational change of p47^ : CW ESR and Four-Pulse DEER study by a site-directed spin labeling (SDSL)(30. Protein function (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Enhanced Induction of Apoptosis by Combined Treatment of Human Carcinoma Cells with X Rays and Death Receptor Agonists
- Apoptosis signal transduction pathway derived from Golgi apparatus injury in light-illuminated HeLa cells in the presence of photosensitizer.
- Induction of Apoptosis through the Activation of SAPK/JNK Followed by the Expression of Death Receptor Fas in X-irradiated Cells
- CYTOTOXIC EFFECT OF ACYCLOVIR ON CULTURED MAMMALIAN CELLS TO WHICH HERPESVIRUS THYMIDINE KINASE GENE WAS INTRODUCED
- Identification of pH-sensitive regions in the mouse prion by the cysteine-scanning spin-labeling ESR technique.
- Magnetic resonance imaging of hepatocellular carcinoma in Long-Evans Cinnamon rats under a magnetic field of 7.05 T
- Spin-trapping detection of superoxides in polymorphonuclear leukocytes stimulated with serum-opsonized zymosan
- The Influence of Gender- and Age-related Differences in the Radiosensitivity of Hematopoietic Progenitor Cells Detected in Steady-state Human Peripheral Blood
- Activation of C-Kit by Stem Cell Factor Induces Radioresistance to Apoptosis through ERK-dependent Expression of Survivin in HL60 Cells
- 1P-277 超音波照射による光分解性ケージド化合物の活性化(放射線生物/活性酸素,第46回日本生物物理学会年会)
- Relationship between cell death and organella damage in H_2O_2 or ^1O_ treated Chinese hamster V79 cells.
- 3L1422 Effects of O-linked N-acetylglucosamine (O-GIcNAc) modification on oxidative stress-response(Cell biology 5,The 49th Annual Meeting of the Biophysical Society of Japan)
- 3C1546 Inositol hexaphosphate and its apatite cements -induced ROS generation and their biological effects on human cells(3C Molecular genetics & Gene expression, Development & Differentation, Radiobiology & Active oxygen,The 49th Annual Meeting of the Bi
- Concentration-Dependent Modes of Cell Death in Chinese Hamster V79 Cells after Treatments with H_2O_2
- Retroviral Sequence Located in Border Region of Short Unique Region and Short Terminal Repeat of Md5 Strain of Mareks Disease Virus Type 1
- 2P-225 MHz超音波によるU937細胞におけるシグナルトランスダクションの発現(放射線生物-活性酸素,第47回日本生物物理学会年会)
- 1P-278 低MHz超音波によるU937細胞への生体影響(放射線生物/活性酸素,第46回日本生物物理学会年会)
- INHIBITION OF DNA REPAIR IN X-IRRADIATED CHINESE HAMSTER V79' CELLS BY NUCLEOSIDE ANALOGUES
- P-20 Reactive oxygen species-independent deformation of copper-centered proteins by 1 MHz ultrasound(Poster Presentations)
- P-19 Effect of OH radicals induced by 1 MHz-ultrasound on HeLa cells(Poster Presentations)
- P-6 Sonochemical cleavage at photosensitive crosslinkage of caged fluorescein derivatives(Poster Presentations)
- Hyper-O-GlcNAcylation Inhibits the Induction of Heat Shock Protein 70 (Hsp 70) by Sodium Arsenite in HeLa Cells