Hyper-O-GlcNAcylation Inhibits the Induction of Heat Shock Protein 70 (Hsp 70) by Sodium Arsenite in HeLa Cells
スポンサーリンク
概要
- 論文の詳細を見る
O-Linked β-N-acetylglucosamine-modification (O-GlcNAcylation) is a reversible, post-translational, and regulatory modification of nuclear, mitochondrial, and cytoplasmic proteins that is responsive to cellular stress. However, the role of O-GlcNAcylation in the induction of heat shock proteins (Hsps) by arsenite remains unclear. We used O-(2-acetamido-2-deoxy-D-glucopyranosylidene) amino N-phenyl carbamate (PUGNAc), an inhibitor of O-GlcNAcase, and glucosamine (GlcN), an enhancer of the hexosamine biosynthesis pathway, or O-GlcNAc transferase (OGT) short interfering RNA (siRNA) to enhance or suppress cellular O-GlcNAcylation levels, respectively, in HeLa cells. The exposure to arsenite increased O-GlcNAcylation and Hsp 70 levels in HeLa cells. However, the pre-treatment with PUGNAc or GlcN, which enhanced O-GlcNAcylation levels, decreased the arsenite-induced expression of Hsp 70. The pre-treatment with OGT siRNA, which suppressed O-GlcNAcylation levels, did not affect the induction of Hsp 70. We then examined the effects of O-GlcNAcylation on the nuclear translocation and phosphorylation of heat shock factor 1 (HSF1), and found that neither the nuclear translocation nor phosphorylation of HSF1 was regulated by O-GlcNAcylation. Finally, Hsp 70 mRNA expression was induced by arsenite, whereas the addition of PUGNAc slightly suppressed its induction. These results indicate that O-GlcNAcylation is related to arsenite-induced Hsp 70 expression, and demonstrated that hyper-O-GlcNAcylation inhibited the induction of Hsp 70 via transcriptional factors instead of HSF1.
著者
-
Miura Yuri
Research Team For Functional Genomics Tokyo Metropolitan Institute Of Gerontology
-
Endo Tamao
Research Team For Functional Genomics Tokyo Metropolitan Institute Of Gerontology
-
Sakurai Yoko
Research Team for Mechanism of Aging, Tokyo Metropolitan Institute of Gerontology
-
Hiraoka Wakako
Department Of Physics Graduate School Of Science And Technology Meiji University
-
Sakurai Yoko
Research Team for Functional Genomics, Tokyo Metropolitan Institute of Gerontology
-
Sato Takatoshi
Research Team for Mechanism of Aging, Tokyo Metropolitan Institute of Gerontology
-
Sakai Ryo
Research Team for Mechanism of Aging, Tokyo Metropolitan Institute of Gerontology
関連論文
- Confirmation of enhanced expression of heme oxygenase-1 gene induced by ultrasound and its mechanism : analysis by cDNA microarray system, real-time quantitative PCR, and Western blotting
- Proteomic Analysis of Two Types of Exosomes in Human Whole Saliva
- 2P227 酸化ストレスによるアポトーシスにおけるO-結合型N-アセチルグルコサミン(O-GlcNAc)修飾の影響(細胞生物的課題(接着,運動,骨格,伝達,膜),第48回日本生物物理学会年会)
- 2P016 ヒトプリオンタンパク質におけるHis96とHis111のCu2+結合能力(蛋白質-構造,第48回日本生物物理学会年会)
- Comparative proteomics of flotillin-rich Triton X-100-insoluble lipid raft fractions of mitochondria and synaptosome from mouse brain
- Proteomic Study on X-irradiation-responsive Proteins and Ageing : Search for Responsible Proteins for Radiation Adaptive Response
- 3P-016 マウスプリオンタンパク質ミュータントの線維形成(蛋白質-構造,第47回日本生物物理学会年会)
- 1P-015 変異マウスプリオンのpH依存性構造変化(蛋白質・構造(1),第46回日本生物物理学会年会)
- Synthesis and Evaluation of Mannosyl Peptides as an Acceptor for N-Acetylglucosaminyltransferase (POMGnT-1)
- CYTOTOXIC EFFECT OF ACYCLOVIR ON CULTURED MAMMALIAN CELLS TO WHICH HERPESVIRUS THYMIDINE KINASE GENE WAS INTRODUCED
- 1P-277 超音波照射による光分解性ケージド化合物の活性化(放射線生物/活性酸素,第46回日本生物物理学会年会)
- Relationship between cell death and organella damage in H_2O_2 or ^1O_ treated Chinese hamster V79 cells.
- Proteomic Approach for Biomarker Discovery in Radioadaptive Responses : Age-Dependent Variations of Cell Response to Low-Dose Radiation
- Survival responses to oxidative stress and aging
- 3L1422 Effects of O-linked N-acetylglucosamine (O-GIcNAc) modification on oxidative stress-response(Cell biology 5,The 49th Annual Meeting of the Biophysical Society of Japan)
- 3C1546 Inositol hexaphosphate and its apatite cements -induced ROS generation and their biological effects on human cells(3C Molecular genetics & Gene expression, Development & Differentation, Radiobiology & Active oxygen,The 49th Annual Meeting of the Bi
- Concentration-Dependent Modes of Cell Death in Chinese Hamster V79 Cells after Treatments with H_2O_2
- 2P-225 MHz超音波によるU937細胞におけるシグナルトランスダクションの発現(放射線生物-活性酸素,第47回日本生物物理学会年会)
- 1P-278 低MHz超音波によるU937細胞への生体影響(放射線生物/活性酸素,第46回日本生物物理学会年会)
- INHIBITION OF DNA REPAIR IN X-IRRADIATED CHINESE HAMSTER V79' CELLS BY NUCLEOSIDE ANALOGUES
- Translocation of Lysosomal Cathepsin D Caused by Oxidative Stress or Proteasome Inhibition in Primary Cultured Neurons and Astrocytes
- P-20 Reactive oxygen species-independent deformation of copper-centered proteins by 1 MHz ultrasound(Poster Presentations)
- P-19 Effect of OH radicals induced by 1 MHz-ultrasound on HeLa cells(Poster Presentations)
- P-6 Sonochemical cleavage at photosensitive crosslinkage of caged fluorescein derivatives(Poster Presentations)
- Hyper-O-GlcNAcylation Inhibits the Induction of Heat Shock Protein 70 (Hsp 70) by Sodium Arsenite in HeLa Cells