Mutation Induction and RBE of Low Energy Neutrons in V79 Cells
スポンサーリンク
概要
- 論文の詳細を見る
We have examined the neutron energy dependency of cell killing and mutation induction at the hprt locus in Chinese hamster V79 cells. Monoenergetic neutrons at 0.32, 0.57, and 1.2 MeV were generated at the Hiroshima University Radiobiological Research Accelerator (HIRRAC) Facility, and were used to irradiate cells. The variation in RBE with neutron energy for the end points of cell survival and hprt mutation induction was observed. When compared to ^<137>Csγ-rays, all neutron energies were more effective at both cell killing and induction of mutation. Over the range of the neutron energies examined, we found that cytotoxicity increased as the energy decreased from 1.2 to 0.32 MeV. In comparison to γ-rays, RBEs for cell lethality at 10% survival were 5.7, 6.7, and 7.6 for 1.2, 0.57, and 0.32 MeV, respectively. Mutation induction, on the other hand, was highest at 0.57 MeV with a gradual decrease at 1.2 and 0.32 MeV. RBEs for mutation induction were 9.7, 19.4, and 13.9 for 1.2, 0.57, and 0.32 MeV neutrons. We isolated independent V79 cell mutants at the hprt locus from untreated and neutron-exposed cells and determined the genetic changes underlying the mutation by multiplex polymerase chain reaction (PCR)- based exon deletion analysis. Preliminary results are suggestive of a specific relationship between deletion patern and neutron energy.
- 日本放射線影響学会の論文
著者
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Komatsu K
Hiroshima Univ. Hiroshima Jpn
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Komatsu Kenshi
Research Institute For Radiation Biology And Medicine Hiroshima University
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ENDO SATORU
Research Institute for Radiation Biology and Medicine, Hiroshima University
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HILL Colin
Department of Microbiology, University College Cork
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INADA Tetsuo
Department of Radiological Sciences, Ibaraki Prefectural University of Health Sciences
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Komatsu K
Department Of Genome Repair Dynamics Radiation Biology Center Kyoto University
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Hill C
Department Of Radiation Oncology University Of Southern California
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Hill Colin
Department Of Radiation Oncology University Of Southern California
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Hill Colin
Department Of Microbiology University College Cork
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遠藤 暁
Mechanical System Engineering Graduate School Of Engineering Hiroshima University
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Endo Satoru
Department Of Biochemistry Cancer Institute Okayama University Medical School
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Inada Tetsuo
Department Of Radiological Sciences Ibaraki Prefectural University Of Health Sciences
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Komatsu Kenshi
Dep. Of Genome Repair Dynamics Radiation Biology Center Kyoto Univ.
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Komatsu Kenshi
Department Of Radiation Biology Research Institute For Radiation Biology And Medicine Hiroshima Univ
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Kubata Nobuo
Ibaraki Prefectural Univ. Of Health Sci.
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Kubota Nobuo
北海道大学 獣医学研究院放射線学教室
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Kubota Nobuo
Pola Chemical Industries Inc.
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Kubota Nobuo
Department Of Radiological Sciences Ibaraki Prefectural University Of Health Sciences
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Okada Shinobu
茨城県立医療大学
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Okada Shinobu
Department Of Pathobiology School Of Nursing Chiba University
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Okada Shinobu
Department Of Radiological Sciences Ibaraki Prefectural University Of Health Sciences
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Okada Shinobu
Department Of Neuropathology Medical Research Institute Tokyo Medical And Dental University
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Ozawa Fujiko
Department Of Radiological Sciences Ibaraki Prefectural University Of Health Sciences
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NAGATOMO SAYAKA
Department of Radiological Sciences, Ibaraki Prefectural University of Health Sciences
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Nagatomo Sayaka
Department Of Radiological Sciences Ibaraki Prefectural University Of Health Sciences
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Kubota Nobuo
Department Of Radiological Sciences. Ibaraki Prefectural University Of Health Sciences
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Endo Satoru
Div. Environ. Biol. Res. Inst. Rad. Biol. And Med. Hiroshima University
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Kubota Nobuo
Department Of Pharmacology School Of Medicine Showa University
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Hill Coline
Human Oncology
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Komatsu Kenshi
Div. Of Environ. Biol. Res. Inst. Rad. Biol. Med. Hiroshima University.
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