Purification and characterization of a novel prolyl endopeptidase from Aspergillus niger
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概要
- 論文の詳細を見る
A novel prolyl endopeptidase has been purified to homogeneity and characterized from the crude extract powder of the culture filtrate of Aspergillus niger var. macrosporus. It was shown to be a monomeric protein with a molecular weight of approximately 54,500, cleaving Pro-X bonds not only in oligopeptides but also in proteins. It has a pH optimum at around 3.7 and is strongly inhibited by diisopropylfluorophosphate, thus being an acid serine endopeptidase. Comparison of the amino acid sequences of related peptidases in the database indicates that it belongs, not to the serine peptidase family S9 which includes prolyl oligopeptidase, but to the serine peptidase family S28 which includes Pro-specific exopeptidases, such as lysosomal Pro-X carboxypeptidase and dipeptidyl peptidase II. (Communicated by Masanori OTSUKA, M.J.A.)
- 日本学士院の論文
著者
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Takahashi K
School Of Life Sciences Tokyo Univ. Of Pharmacy And Life Sciences
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Takahashi Kayoko
Central Research Lnstitute Meiji Milk Products Co. Ltd.
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Takahashi K
Tokyo Univ. Agriculture And Technol. Tokyo Jpn
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Tanokura M
Univ. Tokyo Tokyo Jpn
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Tanokura Masaru
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences Univers
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Tanokura Masaru
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Kubota Keiko
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences Univers
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TAKAHASHI Kenji
Laboratory of Radiological Technology, Hirosaki University School of Health Sciences
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KUBOTA Keiko
Laboratory of Molecular Biochemistry, School of Life Science, Tokyo University of Pharmacy and Life
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Kubota K
School Of Life Sci. Tokyo Univ. Of Pharmacy And Life Sci.
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Takahashi Ken-ichi
Department Of Industrial Chemistry Faculty Of Engineering Chiba Institute Of Technology
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Miyakawa Takuya
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences Univers
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Kubota Keiko
Laboratory Of Molecular Biochemistry School Of Life Sciences Tokyo University Of Pharmacy And Life S
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Takahashi Kenji
Laboratory Of Molecular Biochemistry School Of Life Science Tokyo University Of Pharmacy And Life Sc
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KUBOTA Keiko
Laboratory of Molecular Biochemistry, School of Life Science, Tokyo University of Pharmacy and Life Science
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TAKAHASHI Kenji
Laboratory of Molecular Biochemistry, School of Life Science, Tokyo University of Pharmacy and Life Science
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