Reduced Immunogenicity of β-Lactoglobulin by Conjugating with Chitosan

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概要

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• Bovine β-lactoglobulin (β-LG) was conjugated with chitosan (CHS) by means of a water-soluble carbodiimide to reduce the immunogenicity of β-LG. Each β-LG-CHS conjugate was purified by ion-exchange chromatography and hydrophobic chromatography. The conjugation between β-LG and CHS was confirmed by SDS–PAGE, the isoelectric point of the conjugate being higher than that of β-LG. Two types of the β-LG-CHS conjugate were obtained with molar ratios of β-LG to CHS of 1:1 (F1) and 1:2 (F2). Structural analyses by fluorescence measurement, ELISA with monoclonal antibodies and retinol-binding activity indicated that the conjugates had almost maintained the native structure of β-LG. The antigenicity of the β-LG-CHS conjugates was similar to that of β-LG in C3H/He mice. Reduction of the immunogemicity of β-LG was achieved by conjugation with CHS. In particular, F2 showed very low immunogenicity. B cell epitopes of β-LG and the conjugates recognized in C3H/He mice were determined with 15-mer multi-pin peptide; the linear epitope profiles of the conjugates were found to be similar to those of β-LG, while the antibody response to each epitope was dramatically reduced. Conjugation of β-LG with chitosan was effective for reducing the immunogenicity of β-LG.

• 社団法人 日本農芸化学会の論文
• 2006-10-23

著者

• Takahashi K

  School Of Life Sciences Tokyo Univ. Of Pharmacy And Life Sciences

• Yoshida Tadashi

  Dep. Of Applied Biological Sci. Tokyo Univ. Of Agriculture And Technol.

• AOKI Tomomi

  Institute of Symbiotic Science and Technology, Division of Agriscience and Bioscience, Tokyo Univers

• ISKANDAR Silvia

  Institute of Symbiotic Science and Technology, Division of Agriscience and Bioscience, Tokyo Univers

• YOSHIDA Tadashi

  Institute of Symbiotic Science and Technology, Division of Agriscience and Bioscience, Tokyo Univers

• TAKAHASHI Koji

  Institute of Symbiotic Science and Technology, Division of Agriscience and Bioscience, Tokyo Univers

• HATTORI Makoto

  Institute of Symbiotic Science and Technology, Division of Agriscience and Bioscience, Tokyo Univers

• Hattori M

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Hattori Makoto

  Dep. Of Applied Biological Sci. Tokyo Univ. Of Agriculture And Technol.

• Iskandar Silvia

  Institute Of Symbiotic Science And Technology Division Of Agriscience And Bioscience Tokyo Universit

• Takahashi K

  Dep. Of Applied Biological Sci. Fac. Of Agriculture Tokyo Univ. Of Agriculture And Technol.

• Aoki Tomomi

  Division Of Agriscience And Bioscience Institute Of Symbiotic Science And Technology Tokyo Universit

• Yoshida Tadashi

  Institute Of Applied Microbiology Faculty Of Agriculture Hokkaido University

• Takahashi Koji

  Institute Of Symbiotic Science And Technology Division Of Agriscience And Bioscience Tokyo University Of Agriculture And Technology

• Hattori Makoto

  Department of Applied Biological Science, Tokyo University of Agriculture and Technology

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