Functional Changes of Lysozyme by Conjugating with Carboxymethyl Dextran
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概要
- 論文の詳細を見る
Hen egg lysozyme-carboxymethyl dextran (HEL-CMD) conjugate was prepared by using water-soluble carbodiimide as a model protein-acidic polysaccharide conjugate for improving the protein function. An acid-amide bond between HEL and CMD was confirmed by SDS-PAGE, isoelectric focusing and IR spectra. The molar ratio of CMD to HEL in the conjugate was 1:1. The isoelectric point of the conjugate was 5.5-6.0, which is much lower than that of HEL. Spectroscopic studies suggested that the conformation around the Trp residue had not changed but the α-helix content had decreased to about 1/3 that for native HEL. The conjugate maintained about 60% of the enzymatic activity of native HEL at 40-60℃, while it was about 1.4 times as active as native HEL at 4℃ and 80℃. The conjugate was more stable to proteolysis than native HEL. The denaturation temperature of the conjugate was about 73℃, which is almost the same as that of native HEL. However, the enthalpy for denaturation of the conjugate was about 1/3 that of native HEL, which corresponds to the decrease in α-helix content.
- 社団法人日本農芸化学会の論文
- 1994-01-23
著者
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TAKAHASHI Koji
Department of Pediatrics, Nihon Kokan Hospital
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Nagasawa Koichi
Department of Internal Medicine Tama-Nagayama Hospital, Nippon Medical School
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HATTORI Makoto
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an
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Nagasawa Koichi
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Imamura Shigeo
Laboratory Of Molecular Genetics College Of Agriculture Ibaraki University
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Hattori M
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Imamura Shigeo
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an
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Hattori Makoto
Department Of Applied Biological Science Tokyo University Of Agriculture And Technology
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Takahashi Koji
Department Of Chemistry And Chemical Engineering Yamagata University
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Hattori Makoto
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Hattori Makoto
Department Of Agricultural Chemistry The University Of Tokyo
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Takahashi Koji
Department Of Aeronautics And Astronautics Kyushu University
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Takahashi Koji
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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TAKAHASHI Koji
Departent of Materials Science and Engineering,Faculty of Engineering,Yamagata University
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