Polymerized Structure and Improved Physical and Biochemical Material Properties of Carboxymethyl Dextran-Conjugated Collagen
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概要
- 論文の詳細を見る
Pepsin-solubilized collagen (PSC) was conjugated with carboxymethyl dextran (CMD) using cyanogen bromide to obtain a PSC-CMD film having improved physical properties, physiological properties, and cell affinity. The conjugation was confirmed by the loss of the α- and β-subunit chains and the polymerized band on SDS–PAGE, and by a decrease in the isoelectric point to 3.2. PSC-CMD had a large polymerized structure with the 6 PSC and 228 CMD molecules. PSC-CMD was readily soluble in water, reconstructed a matrix with a less-ordered structure and a characteristic morphological shape, and lost platelet aggregation-inducing ability. The PSC-CMD film, cross-linked by ultraviolet irradiation, exhibited reduced solubility, moderate water vapor permeability, and increased flexibility. PSC-CMD coatings exhibited good cell attachment and growth for fibroblasts and vein endothrical cells.
- 社団法人 日本農芸化学会の論文
- 2008-06-23
著者
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TAKAHASHI Koji
Department of Pediatrics, Nihon Kokan Hospital
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Yoshida Tadashi
Dep. Of Applied Biological Sci. Tokyo Univ. Of Agriculture And Technol.
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YOSHIMURA Keiji
Tokyo Metropolitan Leather Technology Center
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Hattori M
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Maeda Toshihiro
Department Of Anatomy Of Shiga University Of Medical Science
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Takahashi K
Dep. Of Applied Biological Sci. Fac. Of Agriculture Tokyo Univ. Of Agriculture And Technol.
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Hattori Makoto
Department Of Applied Biological Science Tokyo University Of Agriculture And Technology
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Yoshida Tadashi
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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OKADA Harumi
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an
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Takahashi Koji
Department Of Chemistry And Chemical Engineering Yamagata University
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Maeda Toshihiro
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Okada Harumi
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Hattori Makoto
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Hattori Makoto
Department Of Agricultural Chemistry The University Of Tokyo
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Takahashi Koji
Department Of Aeronautics And Astronautics Kyushu University
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Yoshida Tadashi
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University:(present Office)frontie
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TAKAHASHI Koji
Departent of Materials Science and Engineering,Faculty of Engineering,Yamagata University
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