Substrate Specificities of Deuterolysin from Aspergillus oryzae and Electron Paramagnetic Resonance Measurement of Cobalt-substituted Deuterolysin(Biochemistry & Molecular Biology)

概要

論文の詳細を見る
The substrate specificities of deuterolysin, a 19-kDa zinc-protease (EC 3. 4. 24. 39) from Aspergillus oryzae, were investigated at pH 9.0 with various fluorogenic acyl-peptide-4-methylcoumaryl-7-amides (peptide-MCAs). N-Butoxycarbonyl-Arg-Val-Arg-Arg-MCA was the best substrate for deuterolysin. We therefore measured its kinetic parameters. Deuterolysin had high activity toward the peptide bonds next to pairs of basic residues in calf thymus histone H4. The specificity of cobalt-substituted deuterolysin (Co-deuterolysin) for peptide-MCAs was similar to that of native deuterolysin. The CD spectrum of Co-deuterolysin was similar to that of the native deuterolysin. The metal coordination sphere of Co-deuterolysin was analyzed by Q-band (33.9570 GHz) electron paramagnetic resonance (EPR) spectroscopy. Using computer simulation of EPR, we found the g principal values to be g_<xx> = 5.20, g_<yy> = 4.75, and g_<zz> = 2.24 ; the metal center was a divalent cobalt ion in a high spin state.
社団法人日本農芸化学会の論文
2003-02-23