Isolation and Characterization of Chitinase from a Flake-chitin Degrading Marine Bacterium, Aeromonas hydrophila H-2330

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概要

• 論文の詳細を見る

• A flake-chitin degrading marine bacterium was isolated and identified as Aeromonas hydrophila. This strain secreted five chitinases and an β-N-acetylglucosaminidase. The main chitinase (Chi-A) was purified and characterized. The optimum pH of Chi-A was 5-8, and the activity was inhibited by Hg^<2+> and Fe^<3+>. Chi-A was different from chitinases of other Aeromonas species with respect to molecular weight (62,000) and insensitivity to monoiodoacetate. The amino-terminal amino acid sequence showed extensive similarity with chitinases from Gram-negative bacteria.

• 社団法人日本農芸化学会の論文
• 1997-01-23

著者

• Hiraga Kazumi

  Department of Applied Biology, Kyoto Institute of Technology

• Sato R

  Graduate School Of Bio-application And Systems Engineering Tokyo University Of Agriculture And Techn

• Sato R

  Univ. Tokyo Tokyo Jpn

• Hiraga K

  Kyoto Inst. Technol. Kyoto Jpn

• Hiraga Kazumi

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Takahashi S

  Akita Res. Inst. Food And Brewing Akita

• Hiraga K

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• SHIMADA MASAHIKO

  Department of Applied Chemistry, Faculty of Engineering, Tohoku University

• TAKAHASHI Saori

  Department of Bioengineering, Akita Research Institute of Food and Brewing (ARIF)

• KITAZAWA Mitsunori

  Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology

• Shou Lee

  Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology

• Sato Ryoichi

  Central Research Institute, Maruha, Tsukuba

• Oda Kohei

  Central Research Institute, Maruha, Tsukuba

• Shou Lee

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Kitazawa M

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Kitazawa Mitsunori

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Oda K

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Shimada M

  Univ. Electro‐communications Tokyo

• Suzuki Takahito

  Biological Laboratory Faculty Of Science Nara Women's University

• Sato Ryoichi

  Central Research Institute Maruha Co.

• Takahashi Saori

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Shimada Masahiko

  Department Of Applied Chemistry Faculty Of Engineering Tohoku University

• Sato Ryoichi

  Central Research Institute Maruha

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