Isolation and Some Properties of Sorbitol Oxidase from Streptomyces sp. H-7775
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概要
- 論文の詳細を見る
A sorbitol oxidase (SOX) was found in the cell-free extract of a strain isolated from soil. The strain was classified and designated as Streptomyces sp. H-7775. SOX is constitutively expressed in the cell. The molecular weight of SOX that purified from the cell-free extract was 45,000. The optimum pH and the K_m for sorbitol were 6.5-7.5 and 0.26 mM, respectively. The prosthetic group was a covalently bound FAD. SOX catalyzed oxidation of D-sorbitol to glucose and hydrogen peroxide without any requirements of exogenous cofactors. SOX did not react with glucose, a reaction product of D-sorbitol. This feature is useful in its application for diagnosis.
- 社団法人日本農芸化学会の論文
- 1997-10-23
著者
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Hiraga Kazumi
Department of Applied Biology, Kyoto Institute of Technology
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ODA Kohei
Department of Applied Biology, Kyoto Institute of Technology
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Hiraga K
Kyoto Inst. Technol. Kyoto Jpn
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Hiraga Kazumi
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Hiraga K
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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KITAZAWA Mitsunori
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
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KANEKO Norihisa
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
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Oda Kohei
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Kitazawa M
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Kitazawa Mitsunori
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Kaneko Norihisa
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Oda K
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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