High Molecular Weight Transglutaminase Inhibitor Produced by a Microorganism (Streptomyces lavendulae Y-200)
スポンサーリンク
概要
- 論文の詳細を見る
Transglutaminases catalyze the cross-linking and amine incorporation of proteins, and are implicated in various biological phenomena such as blood clotting, wound healing, apoptosis, and cell differentiation. Streptomyces lavendulae Y-200,isolated from soil, produced a substance that inhibited transglutaminases. The inhibitory substance was purified from the cultured medium by procedures of acid precipitation, deoxyribonuclease treatment, and gel filtration chromatography. The partially purified sample was dark brown. The inhibitory activity was stable under acidic, alkaline, and high temperature conditions, and resistant to the treatment with proteinases such as trypsin and Pronase. The molecular weight of the inhibitory substance was estimated to be between 10^4 and 10^5 from its permeability through ultrafilter membranes. The acid hydrolysate of the inhibitory substance contained amino acids and sugars. The inhibitory substance inhibited both calcium-dependent and calcium-independent transglutaminases in a competitive manner with a glutamine substrate. The extent of inhibition caused by the calcium-dependent transglutaminase increased with increasing calcium concentration. The results obtained here may help identify a novel regulatory substance of transglutaminase in biological systems.
- 社団法人日本農芸化学会の論文
- 2000-01-23
著者
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ODA Kohei
Department of Applied Biology, Kyoto Institute of Technology
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NATSUKA Shunji
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
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IKURA Koji
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
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MINAMI Kazuo
Department of Electrical Engineering Nagoya University
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NAKAKITA Shin-ichi
Department of Chemistry, Graduate School of Science, Osaka University
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Natsuka S
Department Of Chemistry Graduate School Of Science Osaka University
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Natsuka Shunji
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Ikura K
Kyoto Inst. Technol. Kyoto Jpn
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Ikura Koji
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Ikura Koji
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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OTOMO Chiemi
Department of Applied Biology, Kyoto Institute of Technology
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HASHIMOTO Hiroyuki
Department of Applied Biology, Kyoto Institute of Technology
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NAKANISHI Katsuyoshi
Department of Applied Biology, Kyoto Institute of Technology
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Oda Kohei
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Oda Kohei
Department Of Applied Biology Kyoto Institute Of Technology
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Ikura K
Department Of Applied Biology Kyoto Institute Of Technology
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Nakakita S
Department Of Chemistry Graduate School Of Science Osaka University
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Otomo Chiemi
Department Of Applied Biology Kyoto Institute Of Technology
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Hashimoto Hiroyuki
Department Of Agricultural Chemistry Kochi University
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Minami Kazuo
Department Of Electrical And Electronic Engineering Niigata University
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Nakakita Shin-ichi
Division Of Functional Glycomics Life Science Research Center Institute Of Research Promotion Kagawa
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Minami Kazuo
Department Of Applied Biology Kyoto Institute Of Technology
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Nakanishi Katsuyoshi
Department Of Applied Biology Kyoto Institute Of Technology
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Hashimoto Hiroyuki
Department Of Applied Biology Kyoto Institute Of Technology
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