β-Galactosidases from Jack Bean and Streptococcus Have Different Cleaving Abilities towards Fucose-Containing Sugars

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概要

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• We examined the sugar-cleaving abilities of β-galactosidases from jack bean and Streptococcus towards sugars containing fucose residues, and found that jack bean β-galactosidase has an ability to cleave the β1-3 linkage between galactose (Gal) and fucose (Fuc) residues, but not β1-4 linkage. On the other hand, streptococcal β-galactosidase was found to cleave the linkage in both Galβ1-4Fuc and Galβ1-3Fuc disaccharide units. Such a difference in sugar-cleaving abilities between these 2 β-galactosidases will be useful for structural analysis of glycans, especially those from species belonging to Protostomia, such as Caenorhabditis elegans.

• 公益社団法人　日本薬学会の論文

著者

• Natsuka Shunji

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Kasai Ken-ichi

  Department Of Biological Chemistry Faculty Of Pharmaceutical Sciences Teikyo University

• Sugiura Ken-ichi

  Department Of Chemistry Tokyo Metropolitan University

• Takeuchi Tomoharu

  Laboratory of Molecular Immunology, Faculty of Pharmaceutical Sciences, Josai University

• Nishiyama Kazusa

  Laboratory of Synthetic Organic and Medicinal Chemistry, School of Pharmaceutical Sciences, Teikyo U

• Takahashi Hideyo

  Laboratory of Synthetic Organic and Medicinal Chemistry, School of Pharmaceutical Sciences, Teikyo U

• Natsugari Hideaki

  Laboratory of Synthetic Organic and Medicinal Chemistry, School of Pharmaceutical Sciences, Teikyo U

• Arata Yoichiro

  Laboratory of Molecular Immunology, Faculty of Pharmaceutical Sciences, Josai University

• Sugiura Ken-ichi

  Department Of Chemistry Graduate School Of Science And Engineering Tokyo Metropolitan University

• Sugiura Ken-ichi

  Department of Biological Chemistry, School of Pharmaceutical Sciences, Teikyo University

• Natsuka Shunji

  Department of Biology, College of Science, Niigata University

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