The DC2.3 Gene in Caenorhabditis elegans Encodes a Galectin That Recognizes the Galactoseβ1→4Fucose Disaccharide Unit

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概要

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• Galectins comprise a large family of β-galactoside-binding proteins in animals and fungi. We previously isolated cDNAs of 10 galectin and galectin-like genes (lec-1 to lec-6 and lec-8 to lec-11) from Caenorhabditis elegans and characterized the carbohydrate-binding properties of their recombinant proteins. In the present study, we isolated cDNA corresponding to an open reading frame of the DC2.3a gene from C. elegans total RNA; this cDNA encodes another potential galectin. A recombinant DC2.3a protein was expressed in Escherichia coli and used for analysis. The protein displayed hemagglutinating activity against rabbit erythrocytes, bound to an asialofetuin-Sepharose column, and was eluted with lactose. Furthermore, frontal affinity chromatography (FAC) analysis confirmed that DC2.3a recognized oligosaccharides with a non-reducing terminal galactose. According to these results, we designated DC2.3 as lec-12. The carbohydrate-binding property of the recombinant DC2.3a/LEC-12a was essentially similar to that of LEC-6. Additionally, DC2.3a/LEC-12a and LEC-6 showed higher affinities for the galactoseβ1→4fucose (Galβ1→4Fuc) disaccharide than for N-acetyllactosamine. This suggests that the principal recognition unit is the Galβ1→4Fuc disaccharide as in the case of the C. elegans galectins. However, the recombinant DC2.3a/LEC-12a showed weak affinity for N-glycan E3, which was previously shown to be a preferential endogenous ligand for LEC-6. The DC2.3a/LEC-12a endogenous ligand structures appear to be somewhat different but contain the same galactose-fucose recognition motif.

著者

• Kasai Ken-ichi

  Department Of Biological Chemistry Faculty Of Pharmaceutical Sciences Teikyo University

• Nemoto-Sasaki Yoko

  Department of Biological Chemistry, Teikyo University School of Pharmaceutical Sciences

• Arata Yoichiro

  Department Of Biological Chemistry Faculty Of Pharmaceutical Sciences Teikyo University

• Nishiyama Kazusa

  Laboratory of Synthetic Organic and Medicinal Chemistry, School of Pharmaceutical Sciences, Teikyo U

• Takahashi Hideyo

  Laboratory of Synthetic Organic and Medicinal Chemistry, School of Pharmaceutical Sciences, Teikyo U

• Natsugari Hideaki

  Laboratory of Synthetic Organic and Medicinal Chemistry, School of Pharmaceutical Sciences, Teikyo U

• Takeuchi Tomoharu

  Department Of Biochemistry Graduate School Of Pharmaceutical Sciences Hokkaido University

• Takai Shunsuke

  Department of Biological Chemistry, School of Pharmaceutical Sciences, Teikyo University

• Yamada Atsushi

  Laboratory of Synthetic Organic and Medicinal Chemistry, School of Pharmaceutical Sciences, Teikyo U

• Yamada Atsushi

  Laboratory of Synthetic Organic and Medicinal Chemistry, School of Pharmaceutical Sciences, Teikyo University

• Nemoto-Sasaki Yoko

  Department of Biological Chemistry, School of Pharmaceutical Sciences, Teikyo University

• Arata Yoichiro

  Department of Biological Chemistry, School of Pharmaceutical Sciences, Teikyo University

• Takeuchi Tomoharu

  Department of Biological Chemistry, School of Pharmaceutical Sciences, Teikyo University

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