Sugar-Binding Properties of the Two Lectin Domains of LEC-1 with Respect to the Galβ1-4Fuc Disaccharide Unit Present in Protostomia Glycoconjugates
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概要
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Galβ1-4Fuc disaccharide unit was recently reported to be the endogenous structure recognized by the galectin LEC-6 isolated from the nematode Caenorhabditis elegans. LEC-1, which is another major galectin from this organism, is a tandem repeat-type galectin that contains two carbohydrate recognition domains, the N-terminal lectin domain (LEC-1Nh) and the C-terminal lectin domain (LEC-1Ch), and was also found to have an affinity for the Galβ1-4Fuc disaccharide unit. In the present study, we compared the binding strengths of LEC-1, LEC-1Nh, and LEC-1Ch to Galβ1-4Fuc, Galβ1-3Fuc, and Galβ1-4GlcNAc units as well as to LEC-6-ligand N-glycans by using frontal affinity chromatography (FAC) analysis. The two lectin domains of LEC-1 exhibited the highest affinity for Galβ1-4Fuc, though sugar-binding properties differed somewhat between LEC-1Nh and LEC-1Ch. Furthermore, these two domains had significantly lower affinities for the LEC-6-binding glycans. These results suggest that the endogenous recognition unit of LEC-1 is likely to be Galβ1-4Fuc, and that the endogenous ligands for LEC-1 are different from those for LEC-6.
著者
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Kasai Ken-ichi
Department Of Biological Chemistry Faculty Of Pharmaceutical Sciences Teikyo University
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Sugiura Ken-ichi
Department Of Chemistry Tokyo Metropolitan University
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Takeuchi Tomoharu
Laboratory of Molecular Immunology, Faculty of Pharmaceutical Sciences, Josai University
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Nishiyama Kazusa
Laboratory of Synthetic Organic and Medicinal Chemistry, School of Pharmaceutical Sciences, Teikyo U
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Takahashi Hideyo
Laboratory of Synthetic Organic and Medicinal Chemistry, School of Pharmaceutical Sciences, Teikyo U
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Natsugari Hideaki
Laboratory of Synthetic Organic and Medicinal Chemistry, School of Pharmaceutical Sciences, Teikyo U
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Arata Yoichiro
Laboratory of Molecular Immunology, Faculty of Pharmaceutical Sciences, Josai University
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Sugiura Ken-ichi
Department Of Chemistry Graduate School Of Science And Engineering Tokyo Metropolitan University
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Takahashi Hideyo
Laboratory of Synthetic Organic and Medicinal Chemistry, School of Pharmaceutical Sciences, Teikyo University
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Natsugari Hideaki
Laboratory of Synthetic Organic and Medicinal Chemistry, School of Pharmaceutical Sciences, Teikyo University
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Sugiura Ken-ichi
Department of Biological Chemistry, School of Pharmaceutical Sciences, Teikyo University
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