Expression of Human Erythropoietin in Cultured Tobacco Cells
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概要
- 論文の詳細を見る
Human erythropoietin (Epo) cDNA was engineered for expression in cultured tobacco cells (Nicotiana tabacum L. cv. BY2). Two plasmid DNAs were constructed : pCEP, which contained Epo cDNA under control of the cauliflower mosaic virus-derived 35S RNA promoter and terminator, and pNSEP, which contained signal sequence-deleted Epo cDNA under control of the 35S RNA promoter and terminator. By using the electroporation method, each of these plasmid DNAs was transferred into the protoplasts of BY2 cells together with a plasmid, pNR35, which conferred G418-resistance on the cells. Four G418-resistant clones were obtained from protoplasts transfected with pNSEP and pNR35, and only one of them, named 11N ; survived in suspension culture. Integration of pNSEP DNA into the genome of 11N cells was confirmed by Southern blot and PCR analyses. Production of Epo mRNA was shown by Northern blot analysis. Epo protein was shown to be expressed in 11N cells by colorimetric enzyme immunoassay. The productivity of Epo in the 11N cells (1 pg/g of wet cells) was very low.
- 社団法人日本農芸化学会の論文
- 1993-08-23
著者
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Matsumoto Shinya
Department of Life Science and Medical Bioscience, Waseda University
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IKURA Koji
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
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ISHII Atsushi
Department of Pediatrics, School of Medicine, Fukuoka University
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Ueda M
Mitsubishi Chemical Co. Ltd.
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Ikura K
Kyoto Inst. Technol. Kyoto Jpn
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Ikura Koji
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Ikura Koji
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Ishii A
Tokai Univ. Kanagawa Jpn
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Ueda Masatsugu
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd.
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Sasaki Ryuzo
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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Ueda M
Life Science Research Institute Snow Brand Milk Products Co. Ltd.
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Ueda M
Shimane Univ. Matsue Jpn
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Matsumoto Shinya
Department Of Environmental Sciences Faculty Of Education And Human Sciences Yokohama National Unive
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Ishii Atsushi
Department Of Applied Biochemistry Faculty Of Engineering Tokai University
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Sasaki Ryuzo
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Matsumoto S
Univ. Tokushima Tokushima‐shi Jpn
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Ueda Masatsugu
Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.
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Matsumoto Shinya
Department Of Applied Physics Faculty Of Engineering Yokohama National University
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Ueda Masatsugu
Research Institute Of Life Science Snow Brand Milk Products
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