A Simple Assay Method for Erythropoietin In Vitro
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概要
- 論文の詳細を見る
A simple in vitro assay method for erythropoietin, a plasma glycoprotein which stimulates erythroid differentiation, has been developed. In this method, a liver cell suspension from 13-day mouse fetus was planted in the wells of a flat-bottomed 96-well microtiter plate and incubated for culturing. The formation of erythroid colonies, which were identified with benzidine staining, was dependent on added erythropoietin. Since this method is inexpensive, sensitive, simple, and only requires a small sample, it could be adopted for assaying the erythropoietin levels in blood, urine, and other materials.
- 社団法人 日本農芸化学会の論文
著者
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CHIBA Hideo
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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Sasaki Ryuzo
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Narita Hiroshi
Department Of Food And Nutrition Kyoto Women's University
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OKADA Hiromu
Department of General Internal Medicine, Kyoto National Hospital
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OKADA Hiromu
Department of Internal Medicine and Clinical Research Institute, Kyoto National Hospital
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NARITA Hiroshi
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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YANAGAWA Shin-ichi
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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ITADA Nobutomo
Clinical Research Institute, Kyoto National Hospital
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