Isolation and Characterization of Opioid Antagonist Peptides Derived from Human Lactoferrin(Food & Nutrition)
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概要
- 論文の詳細を見る
Peptides with affinity for opioid receptors were found in an artificially methyl-esterified peptic digest of human lactoferrin. Three active peptides were purified by two steps of reverse-phase high-performance liquid chromatography. Their structures were Tyr-Leu-Gly-Ser-Gly-Tyr-OCH_3, Arg-Tyr-Tyr-Gly-Tyr-OCH_2, and Lys-Tyr-Leu-Gly-Pro-Gln-Tyr-OCH_3, which respectively correspond to the methyl esters of residues 318-323, 536-540, and 673-679 of human lactoferrin. The IC_<50> values of these peptides were 15,10 and 23 μM, respectively, in a radioreceptor assay in the presence of 1 nM [^3H]naloxone. In the myenteric plexus preparation of the longitudinal muscle of guinea pig ileum, the individual peptides had no opioid agonist activities, but they antagonized [Met^5]enkephalin and morphiceptin when they were at a concentration of 10^<-6>-10^<-5> M, suggesting that these were the opioid antagonist peptides. These three opioid antagonist peptides were named lactoferroxin A, B and C, after casoxin, the opioid antagonist peptide derived from bovine κ-casein. Concerning the antagonist activities of lactoferroxins for opioid receptor sub-types, lactoferroxin A showed preference for μ-receptors, while lactoferroxin B and C had somewhat higher degrees of preference for κ-receptors than for μ-receptors. A study of the structure-activity relationship of the three lactoferroxins and their synthetic analogues showed that these opioid antagonist peptides derived from food protein could be expressed by the general formula X_A-Tyr-X_B-Tyr-OCH_3. An amino acid in position X_A may affect the specificity of the antagonist peptide for opioid receptor sub-types.
- 社団法人日本農芸化学会の論文
- 1990-07-23
著者
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Tani Fumito
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Tani Fumito
Research Institute For Food Science Kyoto University
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Tani F
Institute For Fundamental Research Of Organic Chemistry Kyushu University
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Fumito Tani
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Tani F
Institute For Materials Chemistry And Engineering Kyushu University
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Tani Fumito
Laboratory Of Food And Environmental Sciences Division Of Food Science And Biotechnology Graduate Sc
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Tani Fumito
Institute For Materials Chemistry And Engineering Kyushu University
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IIO Kunihiko
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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CHIBA Hideo
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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YOSHIKAWA Masaaki
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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Chiba H
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University:(present Office)la
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Iio Kunihiko
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University:(present Office)in
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Yoshikawa M
Himeji Inst. Technology Hyogo Jpn
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Yoshikawa Masaaki
Department Of Clinical Pharmacology School Of Medicine Tokai University
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