Purification and Characterization of an Opioid Antagonist from a Peptic Digest of Bovine к-Casein
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概要
- 論文の詳細を見る
A chloroform/methanol extract of a peptic digest of bovine K-casein had binding activity to opioid receptors of rat brain. The active peptide was isolated by reverse-phase liquid chromatography on ODS and CN columns. The structure of the peptide was Ser-Arg-Tyr-Pro-Ser-Tyr-OCH3, which corresponds to the methyl ester of the 33 - 38th residues of κ-casein. The peptide was esterified during the extraction process, and the structure was confirmed by a chemical synthesis. The IC50 value of the peptide in a radioreceptor assay in the presence of 1 HM [3H]naloxone was 15 μM. The peptide did not have opioid agonistic activities in any of the assay systems tested. In the guinea pig ileum and rabbit vas deferens preparations, however, the peptide antagonized with morphiceptin and dynorphin A-(1-13), respectively. The peptide did not antagonize with [Leu]enkephalin in the mouse vas deferens preparations. Therefore, the peptide was an antagonist selective for the μ- and κ-types of opioid receptors. The peptide was named casoxin.
- 社団法人日本農芸化学会の論文
- 1986-11-23
著者
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Tani Fumito
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Tani Fumito
Research Institute For Food Science Kyoto University
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Tani F
Institute For Fundamental Research Of Organic Chemistry Kyushu University
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Fumito Tani
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Tani F
Institute For Materials Chemistry And Engineering Kyushu University
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Tani Fumito
Laboratory Of Food And Environmental Sciences Division Of Food Science And Biotechnology Graduate Sc
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Tani Fumito
Institute For Materials Chemistry And Engineering Kyushu University
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CHIBA Hideo
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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YOSHIKAWA Masaaki
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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ASHIKAGA Tadaaki
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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TOSHIMURA Takashi
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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Chiba H
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University:(present Office)la
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Yoshikawa M
Himeji Inst. Technology Hyogo Jpn
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Yoshikawa Masaaki
Department Of Clinical Pharmacology School Of Medicine Tokai University
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Ashikaga Tadaaki
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Toshimura Takashi
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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