Formation of Enzyme-EnzymeHeteroconjugates
スポンサーリンク
概要
- 論文の詳細を見る
Enzyme-enzyme heteroconjugates of phosphoglyceromutase, enolase and pyruvate kinase, which sequentially act to produce ATP in the glycolytic pathway, were constructed with a bifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The conjugates of phosphoglyceromutase- enolase, enolase-pyruvate kinase and phosphoglyceromutase-enolase-pyruvate kinase were purified so as to be free from free forms of enzymes and properties of the conjugates were investigated in terms of overall reactions, and Km values and heat stability of each enzyme in the conjugates. When the initial phase of the overall reaction catalysed by a conjugate was compared with that by a mixture of the free enzymes, it was found that the lag time required for the system to achieve a steady-state was reduced in the conjugate system. This was true for all conjugates constructed. Nosignificant increases in the Km values of the enzymesin conjugates were noted. Improvement of stability against heat was not observed for any conjugates.
- 社団法人 日本農芸化学会の論文
著者
-
Ikura Koji
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
-
CHIBA Hideo
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
-
Sasaki Ryuzo
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
-
OKUMURA Katsuzumi
Department of Bioscience, Faculty of Bioresources, Mie University
関連論文
- Method for Purification of Fluorescence-Labeled Oligosaccharides by Pyridylamination(Biochemistry & Molecular Biology)
- Comparative Biochemical Study of N-Linked Glycans from Skin of a Squid, Todarodes pacificus
- Characterization of Wheat Germ Agglutinin Ligand on Soluble Glycoproteins in Caenorhabditis elegans
- Inhibition of Transglutaminase by Synthetic Tyrosine Melanin(Biochemistry & Molecular Biology)
- Structural Analysis of N-Linked Glycans in Caenorhabditis elegans
- High Molecular Weight Transglutaminase Inhibitor Produced by a Microorganism (Streptomyces lavendulae Y-200)
- Expression of Human Erythropoietin in Cultured Tobacco Cells
- Gelation of Bean 11S Globulins by Ca^ -Independent Transglutaminase
- Characterization of Erythropoietin Isolated from Rat Serum : Biochemical Comparison of Rat and Human Erythropoietins
- Analysis of Catalytic Action of Transglutaminase Induced in Human Promyelocytic Leukemia (HL-60) and Human Hepatoblastoma (HepG2) Cells
- Isolation and Characterization of Opioid Antagonist Peptides Derived from Human Lactoferrin(Food & Nutrition)
- Purification and Characterization of an Opioid Antagonist from a Peptic Digest of Bovine к-Casein
- Opioid Peptides from Milk Proteins
- Characterization of Human Recombinant Transglutaminase 1 Purified from Baculovirus-infected Insect Cells
- GTP, an Inhibitor of Transglutaminases, is Hydrolyzed by Tissue-type Transglutaminase (TGase 2) but not by Epidermal-type Transglutaminase (TGase 3)
- Characterization og Recombinant Mouse Epidermao-Type Transglutaminase (TGase3) : Regulation of Its Activity by Proteolysis and Guanine Nucleotides
- Potentiation of the Antihypertensive Activity of Orally Administered Ovokinin, a Vasorelaxing Peptide Derived from Ovalbumin, by Emulsification in Egg Phosphatidyl-choline
- Effects of Protein Disulfide-isomerase on the Refolding of Human Pro-urokinase Cloned and Expressed in Escherichia coli(Biological Chemistry)
- Increase Caused by Interleukin-6 in Promoter Activity of Guinea Pig Liver Trans-glutaminase Gene
- Secretory Production of Erythropoietin and the Extracellular Domain of the Erythropoietin Receptor by Bacillus brevis : Affinity Purification and Characterization
- Expression of Bovine α_-casein cDNA in Escherichia coli(Food & Nutrition)
- Cloning of cDNA Coding for Guinea Pig Liver Transglutaminase(Biological Chemistry)
- Effects of Burst-promoting Activity and Erythropoietin from Human Urine on Erythroid Burst Forming Units in Human Peripheral Blood(Biological Chemistry)
- Monoclonal Antibody to Noncatalytic Subunit of Bovine Plasma Factor XIII: Selective Isolation of Catalytic Subunit(Biological Chemistry)
- Identification of New Amine Acceptor Protein Substrate Candidates of Transglutaminase in Rat Liver Extract : Use of 5-(Biotinamido) Pentylamine as a Probe
- Immunohistochemical study of characteristics of bile duct dysplasia evaluated on the basis of expression of metastasis/invasion-related factors and p53
- Overexpression of Transglutaminase in the Drosophila Wing Imaginal Disc Induced an Extra Wing Crossvein Phenotype
- Pleiotropic Functions of Erythropoietin
- Hyper-Phosphorylated Retinoblastoma Protein Suppresses Telomere Elongation
- Complement C3a Agonist Peptides Derived From Proteins
- Targeted Overexpression of Drosophila Transglutaminase-B Induced Characteristic Phenotypes in a Manner Similar to Transglutaminase-A
- Candida guilliermondii H-404株の生産するα-ガラクトシダーゼを用いたα-ガラクトシル基を含む糖質の合成
- Targeted Overexpression of Drosophila Transglutaminase-B Induced Characteristic Phenotypes in a Manner Similar to Transglutaminase-A
- Molecular Cloning and Expression of Caenorhabditis elegans ERp57-Homologue with Transglutaminase Activity
- Cloning of cDNA Sequence Coding for Bovine αs1-Casein
- Effects of Weaning and Resuckling on Casein Messenger RNA Levels in the Rat Mammary Gland
- Isolation and Sequence Analysis of Bovine αs1-Casein cDNA Clone
- Production of Monoclonal Antibodies to Guinea Pig Liver Transglutaminase
- Formation of Enzyme-EnzymeHeteroconjugates
- Incorporation of Amino Acids into Food Proteins by Transglutaminase
- Incorporation of Lysyldipeptides into Food Protein by Transglutaminase
- Effects of Monoclonal Antibody on Guinea Pig Liver Transglutaminase Activity
- A Simple Assay Method for Erythropoietin In Vitro
- Transglutaminase-catalysed Formation of Coenzymatically Active NAD+ Analog • Casein Conjugates
- Partial Purification and Characterization of Rat Mammary Gland Lectin
- Effects of Chemical Phosphorylation of Bovine Casein Components on the Properties Related to Casein Micelle Formation
- Conversion of β-Casein to a Stabilizer for αS1-Casein by Enzymatic and Chemical Modifications
- Preparation of Soluble-insoluble Interconvertible Enzymes : Enzyme•Polymerized αs1-Casein Conjugates
- Post-translational Modification of K>Casein in the Golgi Apparatus of Mid-lactating MammaryGlands from Rat and Cow
- Fractionation and Characterization of 9 Subcomponents of Bovine κ-Casein A
- Dietary Protein-induced Changes of the Erythropoietin Level in Rat Serum
- Nucleotide Sequence Comparison of mRNAs Coding for Major Calcium-sensitive Caseins between Cow and Rat
- Purification and Characterization of UDP-N-Acetylgalactosamine: κ-Casein Polypeptide N-Acetylgalactosaminyltransferase from Mammary Gland of Lactating Cow
- Synthesis of Rat Casein Antigen in Escherichia coli
- Isolation and Characterization of mRNA from MammaryGland of Lactating Cow
- Chemical Characteristics of Bovine Casein Micelles Fractionated by Size on CPG-10/3000 Chromatography